• Product name

  • Description

    Goat polyclonal to ADAM17
  • Host species

  • Specificity

    This antibody is expected to recognise isoform 1 (represented by NP_003174) and not isoform 2 (represented by NP_068604).
  • Tested applications

    Suitable for: IP, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chinese hamster
  • Immunogen

    Synthetic peptide: LQRQNRVDSKETEC, corresponding to C terminal amino acids 811-824 of Human ADAM17.

  • Positive control

    • Hela Lysate
  • General notes

    GenBank Accession Number - NP_003174



Our Abpromise guarantee covers the use of ab13535 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ICC/IF Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Detects a band of approximately 125-130 kDa (predicted molecular weight: 95 , when glycosylated 130 kDa).Can be blocked with Human ADAM17 peptide (ab23216) or ADAM17 peptide (ab7881).


  • Function

    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • Tissue specificity

    Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain

    Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational

    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all


  • Anti-ADAM17 antibody (ab13535) at 0.2 µg/ml + Hela lysate (RIPA buffer, 35µg total protein per lane)

    Predicted band size: 95 , when glycosylated 130 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

    Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
  • HT1080 cells incubated with ab13535 at 10µg/ml for 1 hour at 22°C. Cells were fixed for 20 minutes in 4% PFA in PBS then permeabilised in 0.1% Triton for 4 minutes

  • ab13535 Immunoprecipitate in human HEK293 whole cell lysate. 2 x 10cells were used to prepare lysates and then incubated with primary antibody (diluted 1/250) and matrix (Protein G) for 16 hours at 4°C. For western blotting an Abcam ab28233 Rabbit polyclonal to ADAM17 was used at dilution at 1/5000.

    See Abreview


This product has been referenced in:

  • Xie Y  et al. Rare mutations of ADAM17 from TOFs induce hypertrophy in human embryonic stem cell-derived cardiomyocytes via HB-EGF signaling. Clin Sci (Lond) 133:225-238 (2019). Read more (PubMed: 30610007) »
  • Liu J  et al. CD9 regulates keratinocyte migration by negatively modulating the sheddase activity of ADAM17. Int J Biol Sci 15:493-506 (2019). Read more (PubMed: 30745837) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A


Merci de nous avoir contactés.

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Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique, nous offrons un remboursement ou un remplacement gratuit (Garantie Abpromise, www.abcam.com/abpromise).

Si vous souhaitez utiliser cet anticorps contre une espèce ou dans une application non testées préalablement, nous pouvons vous offrir une offre de test :

1. Nous informer que vous souhaitez tester un de nos anticorps avec une espèce ou dans une application encore non-testée et nous vous enverrons un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,
2. Acheter l'anticorps,
3. Nous envoyer vos résultats (positifs ou négatifs) obtenus avec une espèce ou dans une application encore non testée sous forme d'Abreview (www.abcam.com/abreviews). Ceci activera le code,
4. Commander gratuitement un anticorps primaire de notre catalogue.

J'espère que ces informations vous aideront. N'hésitez pas à me contacter si vous avez besoin de plus de renseignements.

Read More
Western blot
Mouse Cell lysate - whole cell (EL4)
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 10 2012

Human Cell lysate - whole cell (HEK293 cells overexpressing ADAM17)
HEK293 cells overexpressing ADAM17
Immuno-precipitation step
Protein G

Abcam user community

Verified customer

Submitted Jun 12 2009

Immunocytochemistry/ Immunofluorescence
Human Cell (HT1080 Cell line)
HT1080 Cell line
Yes - 0.1% Triton X-100

Abcam user community

Verified customer

Submitted Jan 02 2009

Western blot
Human Cell lysate - other (Immunoprecipitate of HT1080 Cells)
Loading amount
100000 cells
Immunoprecipitate of HT1080 Cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Dec 15 2008

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