Anti-ADAM17 antibody (ab2051)

Rabbit polyclonal ADAM17 antibody. Validated in WB, IHC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 36 publication(s). Independently reviewed in 9 review(s).

Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAM17
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human ADAM17 aa 807-823.
    Sequence:

    ASFKLQRQNRVDSKETE


    (Peptide available as ab7881)

  • Positive control
    • HeLa whole cell lysate, or Jurkat whole cell lysate. This antibody gave a positive result in IHC in the following FFPE tissue: Human pancreas adenocarcinoma.
  • General notes
    TNFa Converting Enzyme.

Properties

Applications

Our Abpromise guarantee covers the use of ab2051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.Can be blocked with ADAM17 peptide (ab7881). Detects bands of 80-130 kDa bands, which may represent mature protein, precursor, and glycosylated TACE.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 10 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 19553533

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
  • Tissue specificity
    Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Domain
    Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
    • A disintegrin and metalloproteinase domain 17 antibody
    • ADA17_HUMAN antibody
    • ADAM 17 antibody
    • ADAM metallopeptidase domain 17 antibody
    • ADAM17 antibody
    • ADAM17 protein antibody
    • CD 156b antibody
    • CD156b antibody
    • CD156b antigen antibody
    • CSVP antibody
    • Disintegrin and metalloproteinase domain-containing protein 17 antibody
    • MGC71942 antibody
    • NISBD antibody
    • NISBD1 antibody
    • Snake venom like protease antibody
    • Snake venom-like protease antibody
    • TACE antibody
    • TNF alpha convertase antibody
    • TNF alpha converting enzyme antibody
    • TNF-alpha convertase antibody
    • TNF-alpha-converting enzyme antibody
    • Tumor Necrosis Factor Alpha Converting Enzyme antibody
    see all

Images

  • Western Blot of HeLa (1, 4), Jurkat (2, 5) and Raji (3, 6) cell lysates labeling ADAM17 with Anti-ADAM17 antibody (ab2051) at 0.5µg/ml in the absence (1-3) or presence of blocking peptide (4-6).
  • All lanes : Anti-ADAM17 antibody (ab2051) at 1/500 dilution

    Lane 1 : HeLa whole cell lysate with absence of blocking peptide
    Lane 2 : Jurkat whole cell lysate with absence of blocking peptide
    Lane 3 : HeLa whole cell lysate with ADAM17 peptide (ab7881)
    Lane 4 : Jurkat whole cell lysate with ADAM17 peptide (ab7881)

    Predicted band size: 93 kDa



    80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.
  • IHC image of ADAM17 staining in Human pancreas adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2051, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Immunofluorescence of TACE in HeLa cells using ab2051 at 10 ug/ml.

  • Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
    Lane 2 : Unrelated antibody of the same subclass as ab2051.

    All lanes : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells

    Secondary
    All lanes : HRP conjugated goat polyclonal antibody

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 93 kDa
    Observed band size: 100,125 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 minute

    See Abreview

  • ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF

  • All lanes : Anti-ADAM17 antibody (ab2051) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : HT1080 cell lysate
    Lane 4 : ES cell lysate
    Lane 5 : L929 cell lysate
    Lane 6 : CHO cell lysate
    Lane 7 : 293T cell lysate
    Lane 8 : ADAM17-overexpressing 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated Goat anti-Rabbit polyclonal antibody at 1/4000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 93 kDa
    Observed band size: 93 kDa
    Additional bands at: 78 kDa (possible isoform)


    Exposure time: 15 seconds

    See Abreview

References

This product has been referenced in:
  • Wawro K  et al. The role of NF-?B and Elk-1 in the regulation of mouse ADAM17 expression. Biol Open 8:N/A (2019). Read more (PubMed: 30709842) »
  • Chenxu G  et al. iRhom2 loss alleviates renal injury in long-term PM2.5-exposed mice by suppression of inflammation and oxidative stress. Redox Biol 19:147-157 (2018). Read more (PubMed: 30165303) »
See all 37 Publications for this product

Customer reviews and Q&As

1-7 of 7 Q&A

Answer

Thank you for your enquiry. The anti-TACE antibody has been tested in mouse lung, brain, liver, kidney, spleen, and 3T3 cells.

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Answer

This antibody cross-reacts with mouse and rat. Other species have not been tested.

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Question
Answer

To the best of our knowledge, this antibody has never been tested in immunoprecipitation, therefore we can't guarantee results.

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Answer

Here is some information regarding our Tace antibody. You may look at our website www.prosci-inc.com if you need more information. If this does not help, please call me at 858-513-2638. Thank you- Steve Reed/ProSci Inc. SOURCE: Rabbit anti-TACE (CT) polyclonal antibody was raised against a peptide corresponding to amino acids 807 to 823 of human TACE (1,2). This sequence differs from those of mouse and rat TACE by one amino acid (3). APPLICATION: This polyclonal antibody can be used for detection of TACE by Western blot at 1:500 to 1:2000 dilution. HeLa or Jurkat whole cell lysate can be used as positive control and 80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated TACE. It is human, mouse, and rat reactive. For research use only.

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Answer

It has not been tested for immunoprecipitation but it should work for ELISA.

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Answer

This antibody contains azide. But we can prepare antibody without azide specially for you. Please let us know hom much do you need. The antibody was generated with a peptide selected at the C-terminus of TACE. Therefore it should recognize both the precursor and the cleaved active form of TACE.

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Answer

We do not have detailed information on the types of Jurkat cells. The cell pellets were from Cellex Bioscience Inc. You may call them at 1-800-325-1112 or 1-612-786-0302.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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