Key features and details
- Rabbit polyclonal to ADAM17
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADAM17 antibody
See all ADAM17 primary antibodies
DescriptionRabbit polyclonal to ADAM17
80 to 130 kDa bands can be detected, which may represent mature protein, precursor, and glycosylated ADAM17.
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
General notesTNFa Converting Enzyme.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Concentration information loading...
PurityImmunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab2051 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.25 - 2 µg/ml. Predicted molecular weight: 93 kDa.Can be blocked with ADAM17 peptide (ab7881).
Detects bands of 80-130 kDa bands, which may represent mature protein, precursor, and glycosylated ADAM17.
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
Tissue specificityUbiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
DomainMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
- Information by UniProt
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
Western Blot of HeLa (1, 4), Jurkat (2, 5) and Raji (3, 6) cell lysates labeling ADAM17 with Anti-ADAM17 antibody (ab2051) at 0.5µg/ml in the absence (1-3) or presence of blocking peptide (4-6).
All lanes : Anti-ADAM17 antibody (ab2051) at 0.25 µg/ml
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : TACE KO HeLa cell lysates
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 93 kDa
Immunofluorescence of ADAM17 in HeLa cells using ab2051 at 10 ug/ml.
Lane 1 : Anti-ADAM17 antibody (ab2051) at 0.5 µg/ml
Lane 2 : Unrelated antibody of the same subclass as ab2051.
All lanes : Human keratinocytes (HaCaT)- whole cell lysate from 20000 cells
All lanes : HRP conjugated goat polyclonal antibody
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 100,125 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
ab2051 at 10µg/ml staining ADAM17 in Hela cells by ICC/IF
ab2051 has been referenced in 45 publications.
- Sun C et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605
- De Rosa MC et al. Characterization of Rare Variants in MC4R in African American and Latino Children With Severe Early-Onset Obesity. J Clin Endocrinol Metab 104:2961-2970 (2019). PubMed: 30811542
- Fei Q et al. Feedback loop in miR-449b-3p/ADAM17/NF-?B promotes metastasis in nasopharyngeal carcinoma. Cancer Med 8:6049-6063 (2019). PubMed: 31433128
- Lian G et al. Macrophage metabolic reprogramming aggravates aortic dissection through the HIF1a-ADAM17 pathway?. EBioMedicine 49:291-304 (2019). PubMed: 31640947
- Wawro K et al. The role of NF-?B and Elk-1 in the regulation of mouse ADAM17 expression. Biol Open 8:N/A (2019). PubMed: 30709842