Western blot abreview for Anti-ADAM17 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (breast)
Loading amount
20 µg
Specification
breast
Gel Running Conditions
Non-reduced Denaturing (4-12% gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
3 hour(s) and 0 minute(s) · Temperature: 20°C

Secondary antibody

Name
Non-Abcam antibody was used: Ge Healthcare HRP secondary Ab
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2500

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: many kDa

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Submitted Feb 10 2011

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