Western blot abreview for Anti-ADAM17 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Various cell lines)
Loading amount
50 µg
Specification
Various cell lines
Gel Running Conditions
Reduced Denaturing (5-20% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%

Other product details

Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: TBST+5%milk

Secondary antibody

Name
Non-Abcam antibody was used: GE Helthcare
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000

Detection

Detection method
ECL
Exposure
15 second(s)
Bands
Specific: 100 kDa
Positive control
HEK293T cells overexpressing human ADAM17

Additional data

Additional Notes
This antibody can detect endogenous levels of ADAM17 from human, mouse and hamster, and produces a specific band with a low background.

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Submitted Mar 02 2011

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