Western blot abreview for Anti-ADAM17 antibody

Good
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (Primary astrocytes)
Loading amount
2 µg
Specification
Primary astrocytes
Gel Running Conditions
Reduced Denaturing (10% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Dilution
1/500
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: anti rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 90 kDa Non-specific: 55 kDa

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Submitted Apr 16 2013

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