Western blot abreview for Anti-ADAM17 antibody

Average
Abreviews
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (10% in TGS)
Sample
Mouse Tissue lysate - other (Whole Hippocampus - Cytosolic and Membrane Fractio)
Specification
Whole Hippocampus - Cytosolic and Membrane Fractio
Blocking step
Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Block Buffer
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Li-Cor IRDye Donkey anti Rabbit 680LT
Host species: Donkey
Clonality: Polyclonal
Conjugation: IRDye­ 680LT
Dilution
1/18000

Detection

Detection method
IR
Bands
Specific: 75-80 kDa Non-specific: 30, 45, 55, kDa
Exposure
5 minute(s) and 30 second(s)

Grant Corbett

Verified customer

Submitted Jul 17 2014

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