Western blot abreview for Anti-ADAM17 antibody

Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human keratinocytes (HaCaT))
Loading amount
20000 cells
Specification
Human keratinocytes (HaCaT)
Blocking step
Milk as blocking agent for 10 minute(s) · Concentration: 5

Other product details

Concentration
0.5 µg/ml
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: Pierce
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 100 and 125 kDa
Negative control
Unrelated antibody of the same subclass.

Additional data

PubMed ID
16735514: View
Additional Notes
ab2051 detected 100 and 125 kDa bands (Lane 1), which may represent mature and pro forms of ADAM17 protein. Lane 2: ab2051 was replaced with an unrelated antibody of the same subclass.

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Submitted Aug 14 2006

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