Anti-ADAM17 antibody (ab39161)

Reviews (3)Q&A (1)References (4)

Key features and details

  • Rabbit polyclonal to ADAM17
  • Reacts with: Rat, Human, Pig
  • Isotype: IgG

Overview

  • Product name

    Anti-ADAM17 antibody
    See all ADAM17 primary antibodies

  • Description

    Rabbit polyclonal to ADAM17

  • Host species

    Rabbit

  • Specificity

    This antibody recognizes ADAM17, but does not react with other ADAMs.

  • Species reactivity

    Reacts with: Rat, Human, Pig

  • Immunogen

    Synthetic peptide based on the propeptide domain of human ADAM17.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41213.)

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Target

  • Function

    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.

  • Tissue specificity

    Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.

  • Domain

    Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
      • A disintegrin and metalloproteinase domain 17 antibody
      • ADA17_HUMAN antibody
      • ADAM 17 antibody
      • ADAM metallopeptidase domain 17 antibody
      • ADAM17 antibody
      • ADAM17 protein antibody
      • CD 156b antibody
      • CD156b antibody
      • CD156b antigen antibody
      • CSVP antibody
      • Disintegrin and metalloproteinase domain-containing protein 17 antibody
      • MGC71942 antibody
      • NISBD antibody
      • NISBD1 antibody
      • Snake venom like protease antibody
      • Snake venom-like protease antibody
      • TACE antibody
      • TNF alpha convertase antibody
      • TNF alpha converting enzyme antibody
      • TNF-alpha convertase antibody
      • TNF-alpha-converting enzyme antibody
      • Tumor Necrosis Factor Alpha Converting Enzyme antibody
      see all

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (4)

    Publishing research using ab39161? Please let us know so that we can cite the reference in this datasheet.

    ab39161 has been referenced in 4 publications.

    • Grieve AG  et al. Phosphorylation of iRhom2 at the plasma membrane controls mammalian TACE-dependent inflammatory and growth factor signalling. Elife 6:N/A (2017). PubMed: 28432785
    • Siggs OM  et al. Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice. Biol Open 3:1151-7 (2014). PubMed: 25395669
    • Brooke MA  et al. iRHOM2-dependent regulation of ADAM17 in cutaneous disease and epidermal barrier function. Hum Mol Genet N/A:N/A (2014). Human . PubMed: 24643277
    • Chavaroche A  et al. Glycosylation of a disintegrin and metalloprotease 17 affects its activity and inhibition. Anal Biochem 449:68-75 (2014). PubMed: 24361716

    Customer reviews and Q&As

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    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADAM17 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Specification
    Intestine
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 25 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM17 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela Cells)
    Specification
    Hela Cells
    Fixative
    Methanol
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 05 2008

    Question

    I have been using your rabbit polyclonal against the propeptide domain of ADAM17 to examine an ADAM17 prep and get no recognition with it at all whereas your other Abs (ab39163 and ab28233) are fine. I think my protein has been cleaved following Arg58 in the propeptide domain and I suspect that your Ab has been made against a peptide within the missing N-terminal 58 amino acids. Is this correct? Also, for the activation site Ab (ab39163) is only the intact site recognised or if after cleavage are both sides recognised or only one?

    Read More
    Answer

    you are correct in assuming that ab39161 recognizes only the prodomain cleaved during ADAM17 maturation. This maturation can be prevented using divalent metal chelators such as EDTA or EGTA. One of the customers who submitted an Abreview for ab39163 noted that "Seems to be selective for Precursor forms of ADAM17". The presence of the 55kDa band as well would indicate that both the cleaved and uncleaved form of this protein are recognized.

    Read More

    Western blot abreview for Anti-ADAM17 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA-MB231 Cells)
    Loading amount
    30000 cells
    Specification
    MDA-MB231 Cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 30 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com