Anti-ADAM17 antibody (ab39161)

Reviews (3)Q&A (1)References (4)

Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab39161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 110 kDa (predicted molecular weight: 93 kDa). 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. The antibody recognizes a 110 kDa band in reduced Western blots of conditioned media or cell lysates, which is rapidly converted to a 55-60 kDa band. Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function

    Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.

  • Tissue specificity

    Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.

  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.

  • Domain

    Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
    Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.

  • Cellular localization

    Membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
      • A disintegrin and metalloproteinase domain 17 antibody
      • ADA17_HUMAN antibody
      • ADAM 17 antibody
      • ADAM metallopeptidase domain 17 antibody
      • ADAM17 antibody
      • ADAM17 protein antibody
      • CD 156b antibody
      • CD156b antibody
      • CD156b antigen antibody
      • CSVP antibody
      • Disintegrin and metalloproteinase domain-containing protein 17 antibody
      • MGC71942 antibody
      • NISBD antibody
      • NISBD1 antibody
      • Snake venom like protease antibody
      • Snake venom-like protease antibody
      • TACE antibody
      • TNF alpha convertase antibody
      • TNF alpha converting enzyme antibody
      • TNF-alpha convertase antibody
      • TNF-alpha-converting enzyme antibody
      • Tumor Necrosis Factor Alpha Converting Enzyme antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-ADAM17 antibody (ab39161)
      Immunocytochemistry/ Immunofluorescence - Anti-ADAM17 antibody (ab39161)Image taken from an anonymous abreview submitted in February 2008

      Methanol fixed HeLa cells incubated with ab39161 at 10 µg/ml for 1 hour at 22°C. Clear membrane staining can be seen. For further details please refer to the Abreview.

    References

    This product has been referenced in:

    • Grieve AG  et al. Phosphorylation of iRhom2 at the plasma membrane controls mammalian TACE-dependent inflammatory and growth factor signalling. Elife 6:N/A (2017). Read more (PubMed: 28432785) »
    • Siggs OM  et al. Genetic interaction implicates iRhom2 in the regulation of EGF receptor signalling in mice. Biol Open 3:1151-7 (2014). Read more (PubMed: 25395669) »
    See all 4 Publications for this product

    Publishing research using ab39161? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-ADAM17 antibody

     Poor
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Intestine)
    Specification
    Intestine
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    None
    Permeabilization
    No
    Blocking step
    Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted May 25 2010

    Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM17 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Hela Cells)
    Specification
    Hela Cells
    Fixative
    Methanol
    Permeabilization
    No
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 05 2008

    Question

    I have been using your rabbit polyclonal against the propeptide domain of ADAM17 to examine an ADAM17 prep and get no recognition with it at all whereas your other Abs (ab39163 and ab28233) are fine. I think my protein has been cleaved following Arg58 in the propeptide domain and I suspect that your Ab has been made against a peptide within the missing N-terminal 58 amino acids. Is this correct? Also, for the activation site Ab (ab39163) is only the intact site recognised or if after cleavage are both sides recognised or only one?

    Read More
    Answer

    you are correct in assuming that ab39161 recognizes only the prodomain cleaved during ADAM17 maturation. This maturation can be prevented using divalent metal chelators such as EDTA or EGTA. One of the customers who submitted an Abreview for ab39163 noted that "Seems to be selective for Precursor forms of ADAM17". The presence of the 55kDa band as well would indicate that both the cleaved and uncleaved form of this protein are recognized.

    Read More

    Western blot abreview for Anti-ADAM17 antibody

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (MDA-MB231 Cells)
    Loading amount
    30000 cells
    Specification
    MDA-MB231 Cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 30 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com