Anti-ADAM17 antibody (ab39162)
Key features and details
- Rabbit polyclonal to ADAM17
- Suitable for: IP, ICC/IF, WB, IHC-P
- Reacts with: Rat, Human, Pig
- Isotype: IgG
Overview
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Product name
Anti-ADAM17 antibody
See all ADAM17 primary antibodies -
Description
Rabbit polyclonal to ADAM17 -
Host species
Rabbit -
Specificity
This antibody recognizes ADAM17, but does not react with other ADAMs. -
Tested applications
Suitable for: IP, ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human, Pig -
Immunogen
Synthetic peptide corresponding to Human ADAM17. Within the cytoplasmic domain of Human ADAM17.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab39162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IP | (1) |
Use at an assay dependent concentration.
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ICC/IF | (2) |
Use at an assay dependent concentration.
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WB | (5) |
Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.
1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
IHC-P | (1) |
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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IP
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 93 kDa. 1/1000 (when using colorimetric substrates such as BCIP/NBT) and 1/5000 (for chemiluminescent substrates). Higher concentrations of antibody may be needed for samples from more distantly related species. Detects a band of approximately 110 kDa in reduced Western blots of conditioned media or cell lysates. (Predicted molecular weight: 93 kDa). Note: EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. |
IHC-P
Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway. -
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6868 Human
- Entrez Gene: 57027 Rat
- Omim: 603639 Human
- SwissProt: P78536 Human
- SwissProt: Q9Z1K9 Rat
- Unigene: 404914 Human
- Unigene: 144585 Rat
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Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Images
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Ab39162 staining human normal colon. Staining is localised to membrane.
Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab39162 has been referenced in 36 publications.
- Zou Z et al. The role of S100B/RAGE-enhanced ADAM17 activation in endothelial glycocalyx shedding after traumatic brain injury. J Neuroinflammation 19:46 (2022). PubMed: 35148784
- Niehues RV et al. The collectrin-like part of the SARS-CoV-1 and -2 receptor ACE2 is shed by the metalloproteinases ADAM10 and ADAM17. FASEB J 36:e22234 (2022). PubMed: 35199397
- Zaruba M et al. ADAM17 Is an Essential Factor for the Infection of Bovine Cells with Pestiviruses. Viruses 14:N/A (2022). PubMed: 35215974
- Ji W et al. Triptolide attenuates inhibition of ankylosing spondylitis-derived mesenchymal stem cells on the osteoclastogenesis through modulating exosomal transfer of circ-0110634. J Orthop Translat 36:132-144 (2022). PubMed: 36185580
- Bunker EN et al. Suppression of a-catenin and adherens junctions enhances epithelial cell proliferation and motility via TACE-mediated TGF-a autocrine/paracrine signaling. Mol Biol Cell 32:348-361 (2021). PubMed: 33378218