Anti-ADAM17 antibody (ab57484)
Key features and details
- Mouse monoclonal to ADAM17
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-ADAM17 antibody
See all ADAM17 primary antibodies -
Description
Mouse monoclonal to ADAM17 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment: RADPDPMKNT CKLLVVADHR FYRYMGRGEE STTTNYLIEL IDRVDDIYRN TSWDNAGFKG YGIQIEQIRI LKSPQEVKPG EKHYNMAKSY PNEEKDAWDV , corresponding to amino acids 215-315 of Human ADAM17
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General notes
This product was changed from ascites to tissue culture supernatant on 25/02/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4 -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab57484 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein. |
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| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | Use at an assay dependent concentration. (methanol fixed cells)
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway. -
Tissue specificity
Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain. -
Domain
Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6868 Human
- Omim: 603639 Human
- SwissProt: P78536 Human
- Unigene: 404914 Human
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Alternative names
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
see all
Images
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Western blot - Anti-ADAM17 antibody (ab57484)
Western blot against tagged recombinant protein immunogen using ab57484 ADAM17 antibody at 1ug/ml. Predicted band size of immunogen is 37 kD
This image was generated using the ascites version of the product.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM17 antibody (ab57484)ADAM17 antibody (ab57484) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
This image was generated using the ascites version of the product.
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Flow Cytometry - Anti-ADAM17 antibody (ab57484)Overlay histogram showing HeLa cells stained with ab57484 (red line). The cells were fixed with methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab57484, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min) used under the same conditions.
Please note that Abcam do not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.This image was generated using the ascites version of the product.
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Western blot - Anti-ADAM17 antibody (ab57484)Image courtesy of an anonymous Abreview.Anti-ADAM17 antibody (ab57484) at 1/1000 dilution + whole cell lysate prepared from MCF7 cells at 100000 cells
Secondary
HRP conjugated sheep anti-mouse polyclonal at 1/2000 dilution
Developed using the ECL technique.
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minuteBlocked with 5% milk for 1 hour at 24°C.
This image was generated using the ascites version of the product.
Protocols
Datasheets and documents
References (11)
ab57484 has been referenced in 11 publications.
- Veit M et al. Mutagenesis of the ADAM17-phosphatidylserine-binding motif leads to embryonic lethality in mice. Life Sci Alliance 2:N/A (2019). PubMed: 31455669
- Jiang C et al. Inactivation of Rab11a GTPase in Macrophages Facilitates Phagocytosis of Apoptotic Neutrophils. J Immunol 198:1660-1672 (2017). PubMed: 28053235
- DeSantis-Rodrigues A et al. ADAM17 Inhibitors Attenuate Corneal Epithelial Detachment Induced by Mustard Exposure. Invest Ophthalmol Vis Sci 57:1687-98 (2016). WB . PubMed: 27058125
- McClurg UL et al. Epithelial cell ADAM17 activation by Helicobacter pylori: role of ADAM17 C-terminus and Threonine-735 phosphorylation. Microbes Infect 17:205-14 (2015). PubMed: 25499189
- Gooden MJ et al. Elevated serum CXCL16 is an independent predictor of poor survival in ovarian cancer and may reflect pro-metastatic ADAM protease activity. Br J Cancer 110:1535-44 (2014). PubMed: 24518602
