Immunocytochemistry/ Immunofluorescence abreview for Anti-ADAM17 antibody

Good
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (H357 (oral SCC))
Specification
H357 (oral SCC)
Fixative
Acetone/methanol
Permeabilization
No
Blocking step
BSA as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C

Other product details

Dilution
1/50
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: PBS

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-mouse IgG FITC
Host species: Donkey
Clonality: Polyclonal
Conjugation: Fluorescein
Dilution
1/100

Additional data

Additional Notes
ADAM17 is lowly expressed in the cells of interest.

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Submitted Jul 03 2008

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