• Product name

  • Description

    Rabbit polyclonal to ADAM17
  • Host species

  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide from the C terminus of human ADAM17 protein.

  • Positive control



Our Abpromise guarantee covers the use of ab75609 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IP: Use at 10µg/mg of lysate.
    WB: Use at a concentration of 5 µg/ml for 2 hrs at RT. Predicted molecular weight: 93 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Cleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
    • Tissue specificity

      Ubiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
    • Sequence similarities

      Contains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
    • Domain

      Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational

      The precursor is cleaved by a furin endopeptidase.
      Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
    • Cellular localization

    • Information by UniProt
    • Database links

    • Alternative names

      • A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
      • A disintegrin and metalloproteinase domain 17 antibody
      • ADA17_HUMAN antibody
      • ADAM 17 antibody
      • ADAM metallopeptidase domain 17 antibody
      • ADAM17 antibody
      • ADAM17 protein antibody
      • CD 156b antibody
      • CD156b antibody
      • CD156b antigen antibody
      • CSVP antibody
      • Disintegrin and metalloproteinase domain-containing protein 17 antibody
      • MGC71942 antibody
      • NISBD antibody
      • NISBD1 antibody
      • Snake venom like protease antibody
      • Snake venom-like protease antibody
      • TACE antibody
      • TNF alpha convertase antibody
      • TNF alpha converting enzyme antibody
      • TNF-alpha convertase antibody
      • TNF-alpha-converting enzyme antibody
      • Tumor Necrosis Factor Alpha Converting Enzyme antibody
      see all


    • Anti-ADAM17 antibody (ab75609) at 5 µg/ml + HeLa cell lysate

      Predicted band size: 93 kDa
      Observed band size: 80 kDa
      why is the actual band size different from the predicted?

    • Detection of ADAM17 by Western Blot of Immunprecipitate.
      Staining of ADAM17 in HeLa cell lysate immunoprecipitated using ab75609 at 10µg/mg lysate.


    This product has been referenced in:

    • Chen AC  et al. Physical and functional interaction between the a- and ?-secretases: A new model of regulated intramembrane proteolysis. J Cell Biol 211:1157-76 (2015). Read more (PubMed: 26694839) »
    • Rice HC  et al. Pancortins interact with amyloid precursor protein and modulate cortical cell migration. Development 139:3986-96 (2012). WB ; Human . Read more (PubMed: 22992957) »
    See all 2 Publications for this product

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