Key features and details
- Rabbit polyclonal to ADAM17 (phospho T735)
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ADAM17 (phospho T735) antibody
See all ADAM17 primary antibodies
DescriptionRabbit polyclonal to ADAM17 (phospho T735)
Specificityab182630 detects endogenous level of ADAM17 only when phosphorylated at Threonine 735.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Extracts from K562 cells treated with UV
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesNon-phospho specific antibodies were removed by chromatography using non-phosphopeptide.
Our Abpromise guarantee covers the use of ab182630 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 93 kDa.|
FunctionCleaves the membrane-bound precursor of TNF-alpha to its mature soluble form. Responsible for the proteolytical release of soluble JAM3 from endothelial cells surface. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 receptor type II, p55 TNF-receptor, transforming growth factor-alpha, L-selectin, growth hormone receptor, MUC1 and the amyloid precursor protein. Also involved in the activation of Notch pathway.
Tissue specificityUbiquitously expressed. Expressed at highest levels in adult heart, placenta, skeletal muscle, pancreas, spleen, thymus, prostate, testes, ovary and small intestine, and in fetal brain, lung, liver and kidney.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
DomainMust be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Only the catalytic domain is essential to shed TNF and p75 TNFR.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Phosphorylated. Stimulation by growth factor or phorbol 12-myristate 13-acetate induces phosphorylation of Ser-819 but decreases phosphorylation of Ser-791.
- Information by UniProt
- A disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme) antibody
- A disintegrin and metalloproteinase domain 17 antibody
- ADA17_HUMAN antibody
ab182630 has been referenced in 2 publications.
- Yoon J et al. A JUN N-terminal kinase inhibitor induces ectodomain shedding of the cancer-associated membrane protease Prss14/epithin via protein kinase CßII. J Biol Chem 295:7168-7177 (2020). PubMed: 32241917
- Sun C et al. ADAM17-regulated CX3CL1 expression produced by bone marrow endothelial cells promotes spinal metastasis from hepatocellular carcinoma. Int J Oncol 57:249-263 (2020). PubMed: 32319605