Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAM19
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Human ADAM19 aa 286-298 (N terminal).
    Sequence:

    RRKLLAQKYHDNA


    Database link: Q9H013

  • Positive control

    • Rat spleen, intestine and brain lysates: HeLa cell lysates: Human lung cancer tissue: Rat spleen tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab191457 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 105 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance

    ADAM19, is a member of the ADAM (a disintegrin and metalloprotease-like domain) family. It has been cloned from mouse and human. ADAM19 was first described in muscle cells as a protein with homology to the fertilins (ADAMs 1 and 2). Initial observations indicated a role for ADAM19 in myoblast fusion, similar to sperm-egg fusion aided by ADAMs 1 and 2. Later works describe ADAM19 in the bone, muscle, lung, heart, brain, kidney, and a wide range of tissues. The Cytoplasmic domain of ADAM19, like ADAMs 9, 12 and 15, contains SH3 ligand domains, which are thought to interact with PKC-d, suggesting specific regulation routes for ADAM19. Also reported is a sequence of ADAM19 lacking the transmembrane and cytoplasmic domains, suggesting that a soluble form is produced. ADAM-19 contains the canonical HExxHxxxxxH zinc metalloproteinase motif, and has been shown to be proteolytically active. Other ADAMs family members (ADAM-10, ADAM-17) have been more thoroughly studied, and are known to play key roles in inflammation, growth factor maturation and release, and a wide range of other functions. The full length ADAM19 sequence codes for a 956 amino acid protein, containing a Type-I transmembrane domain, with a predicted mass is 105 kD. Two shorter sequences have been reported: a 918 amino acid sequence that differs at the carboxyterminal end, and the soluble form, a 538 amino acid version with predicted mass of 59.9 kD. Mouse ADAM19 sequence is 920 AA, predicted at 100.86 kD.
  • Cellular localization

    Cell Membrane. Type I membrane protein.
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase domain 19 (meltrin beta) antibody
    • A disintegrin and metalloproteinase domain 19 antibody
    • ADAM 19 antibody
    • ADAM metallopeptidase domain 19 antibody
    • Disintegrin and metalloproteinase domain containing protein 19 antibody
    • FKSG 34 antibody
    • FKSG34 antibody
    • MADDAM antibody
    • Meltrin beta antibody
    • Metalloprotease and disintegrin dentritic antigen marker antibody
    • Metalloprotease disintegrin meltrin beta antibody
    • MLTN B antibody
    • MLTNB antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling ADAM19 with ab191457 at 1 µg/ml.

  • Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue labeling ADAM19 with ab191457 at 1 µg/ml.

  • All lanes : Anti-ADAM19 antibody (ab191457) at 0.5 µg/ml

    Lane 1 : Rat spleen tissue lysate
    Lane 2 : Rat intestine tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : HeLa cell lysate

    Predicted band size: 105 kDa

References

ab191457 has not yet been referenced specifically in any publications.

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