Overview

  • Product name

    Anti-ADAM2 antibody [EP15281]
  • Description

    Rabbit monoclonal [EP15281] to ADAM2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ADAM2 aa 550-700. The exact sequence is proprietary.
    Database link: Q99965

  • Positive control

    • Human testis lysate; Human testis tissue.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab193668 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 82 kDa).
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Sperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.
  • Tissue specificity

    Expressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Domain

    A tripeptide motif (FEE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
  • Post-translational
    modifications

    The prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase domain 2 antibody
    • ADAM 2 antibody
    • ADAM metallopeptidase domain 2 antibody
    • Adam2 antibody
    • ADAM2_HUMAN antibody
    • AI323749 antibody
    • Cancer/testis antigen 15 antibody
    • CRYN1 antibody
    • CRYN2 antibody
    • CT15 antibody
    • Disintegrin and metalloproteinase domain-containing protein 2 antibody
    • Fertilin beta antibody
    • Fertilin subunit beta antibody
    • Ftnb antibody
    • PH 30 antibody
    • PH 30b antibody
    • PH-30 antibody
    • PH30 antibody
    • Ph30 beta antibody
    • PH30-beta antibody
    see all

Images

  • Anti-ADAM2 antibody [EP15281] (ab193668) at 1/1000 dilution + Human testis lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 82 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ADAM2 with ab193668 at 1/1000 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

References

This product has been referenced in:

  • Peng XG  et al. Evolution of correlation between Helicobacter pylori infection and autoimmune liver disease. Exp Ther Med 14:1487-1490 (2017). Read more (PubMed: 28810614) »
See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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