Key features and details
- Rabbit monoclonal [EP15281] to ADAM2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADAM2 antibody [EP15281]
DescriptionRabbit monoclonal [EP15281] to ADAM2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment within Human ADAM2 aa 550-700. The exact sequence is proprietary.
Database link: Q99965
- Human testis lysate; Human testis tissue.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab193668 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/2000. Detects a band of approximately 100 kDa (predicted molecular weight: 82 kDa).|
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
FunctionSperm surface membrane protein that may be involved in sperm-egg plasma membrane adhesion and fusion during fertilization. Could have a direct role in sperm-zona binding or migration of sperm from the uterus into the oviduct. Interactions with egg membrane could be mediated via binding between its disintegrin-like domain to one or more integrins receptors on the egg. This is a non catalytic metalloprotease-like protein.
Tissue specificityExpressed specifically in spermatogenic cells in the seminiferous cells. Not detected in fetal tissues.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
DomainA tripeptide motif (FEE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
modificationsThe prodomain and the metalloprotease domain are cleaved during the epididymal maturation of the spermatozoa.
- Information by UniProt
- A disintegrin and metalloproteinase domain 2 antibody
- ADAM 2 antibody
- ADAM metallopeptidase domain 2 antibody
Anti-ADAM2 antibody [EP15281] (ab193668) at 1/1000 dilution + Human testis lysate at 20 µg
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 82 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Blocking/Dilution buffer: 5% NFDM/TBST.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling ADAM2 with ab193668 at 1/1000 dilution (1μg/ml), followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
ab193668 has been referenced in 1 publication.
- Peng XG et al. Evolution of correlation between Helicobacter pylori infection and autoimmune liver disease. Exp Ther Med 14:1487-1490 (2017). PubMed: 28810614