Key features and details
- Rabbit polyclonal to ADAM20
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADAM20 antibody
See all ADAM20 primary antibodies
DescriptionRabbit polyclonal to ADAM20
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Fusion protein corresponding to Human ADAM20 (internal sequence). Gene Accssion: BC025378.
Database link: O43506
- PC3 and HeLa cell lysates; Human brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab197829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/25 - 1/100.|
|WB||1/100 - 1/500. Predicted molecular weight: 82 kDa.|
FunctionMay be involved in sperm maturation and/or fertilization.
Tissue specificityTestis specific.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
DomainA tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsHas no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
- Information by UniProt
- A disintegrin and metalloproteinase domain 20 antibody
- A disintegrin and metalloproteinase domain 20 preproprotein antibody
- ADA20_HUMAN antibody
All lanes : Anti-ADAM20 antibody (ab197829) at 1/100 dilution
Lane 1 : PC3 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 40 µg per lane.
Predicted band size: 82 kDa
Exposure time: 10 seconds
Gel: 8% SDS-PAGE
Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADAM20 with ab197829 at 1/30.
ab197829 has not yet been referenced specifically in any publications.