Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAM20
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Fusion protein corresponding to Human ADAM20 (internal sequence). Gene Accssion: BC025378.
    Database link: O43506

  • Positive control

    • PC3 and HeLa cell lysates; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab197829 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/25 - 1/100.
WB 1/100 - 1/500. Predicted molecular weight: 82 kDa.

Target

  • Function

    May be involved in sperm maturation and/or fertilization.
  • Tissue specificity

    Testis specific.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Domain

    A tripeptide motif (VGE) within disintegrin-like domain could be involved in the binding to egg integrin receptor and thus could mediate sperm/egg binding.
    The cysteine-rich domain encodes putative cell-fusion peptides, which could be involved in sperm-egg fusion.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Has no obvious cleavage site for furin endopeptidase, suggesting that the proteolytic processing is regulated.
  • Cellular localization

    Membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase domain 20 antibody
    • A disintegrin and metalloproteinase domain 20 preproprotein antibody
    • ADA20_HUMAN antibody
    • ADAM 20 antibody
    • ADAM metallopeptidase domain 20 antibody
    • ADAM20 antibody
    • Disintegrin and metalloproteinase domain-containing protein 20 antibody
    • OTTHUMP00000236151 antibody
    see all

Images

  • All lanes : Anti-ADAM20 antibody (ab197829) at 1/100 dilution

    Lane 1 : PC3 cell lysate
    Lane 2 : HeLa cell lysate

    Lysates/proteins at 40 µg per lane.

    Predicted band size: 82 kDa


    Exposure time: 10 seconds


    Gel: 8% SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling ADAM20 with ab197829 at 1/30.

References

ab197829 has not yet been referenced specifically in any publications.

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