Key features and details
- Rabbit polyclonal to ADAM22
- Suitable for: WB, IHC-Fr
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ADAM22 antibody
See all ADAM22 primary antibodies
DescriptionRabbit polyclonal to ADAM22
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Human
- WB: Human brain tissue extract, U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. IHC-Fr: Embryonic mouse brain tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab227546 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 100 kDa.
Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature.
|IHC-Fr||1/100 - 1/1000.|
FunctionProbable ligand for integrin in the brain. This is a non catalytic metalloprotease-like protein. Involved in regulation of cell adhesion and spreading and in inhibition of cell proliferation. Neuronal receptor for LGI1.
Tissue specificityHighly expressed in the brain and in some high-grade but not low-grade gliomas. Detected slightly or not at all in other tissues.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain.
modificationsThe precursor is cleaved by a furin endopeptidase.
- Information by UniProt
- A disintegrin and metalloproteinase domain 22 antibody
- ADA22_HUMAN antibody
- ADAM 22 antibody
All lanes : Anti-ADAM22 antibody (ab227546) at 1/500 dilution
Lane 1 : U87-MG whole cell extract
Lane 2 : SK-N-SH whole cell extract
Lane 3 : IMR32 whole cell extract
Lane 4 : SK-N-AS whole cell extract
Lysates/proteins at 30 µg per lane.
All lanes : HRP-conjugated anti-rabbit IgG antibody at 1/10000 dilution
Predicted band size: 100 kDa
10% SDS gel
Running condition: 80V, 15min; 140V, 40 min
Primary antibody incubation: 4C overnight
Washing condition: 5 mL TBST, 4 x 5 minutes
Anti-ADAM22 antibody (ab227546) at 1/500 dilution + Human brain tissue extract at 30 µg
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 100 kDa
Frozen sectioned embryonic mouse brain (mE18.5) stained for ADAM22 with ab227546 (1/500 dilution) (red) in immunohistochemical analysis. Counterstains: DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab227546 has been referenced in 1 publication.
- Li J et al. miR-449a Suppresses Tamoxifen Resistance in Human Breast Cancer Cells by Targeting ADAM22. Cell Physiol Biochem 50:136-149 (2018). PubMed: 30278449