Anti-ADAM23 antibody (ab28304)

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Key features and details

  • Rabbit polyclonal to ADAM23
  • Suitable for: ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

Overview

  • Product name

    Anti-ADAM23 antibody
    See all ADAM23 primary antibodies

  • Description

    Rabbit polyclonal to ADAM23

  • Host species

    Rabbit

  • Specificity

    ab28304 recognises the Propeptide domain of ADAM23.

  • Tested applications

    Suitable for: ICC/IFmore details

  • Species reactivity

    Reacts with: Rat, Human

  • Immunogen

    Synthetic peptide corresponding to the Propeptide of Human ADAM23.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41222.)

  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab28304 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
  • Application notes
    ICC/IF: Use at a concentration of 1 µg/ml.
    WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 92 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      ADAM23 is a non-catalytic metalloprotease-like protein. It is highly expressed in the brain, primarily in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole, and weakly expressed in the heart. 3 isoforms are produced by alternative splicing, alpha, beta and gamma. ADAM23 may play a role in cell-cell and cell-matrix interactions.

    • Cellular localization

      Single pass type I membrane protein. 3 isoforms produced by alternative splicing, gamma isoform is secreted.

    • Database links

      • Alternative names

        • A disintegrin and metalloproteinase domain 23 antibody
        • ADAM metallopeptidase domain 23 antibody
        • Disintegrin and metalloproteinase domain containing protein 23 antibody
        • MDC 3 antibody
        • MDC3 antibody
        • Metalloproteinase like disintegrin like and cysteine rich protein 3 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-ADAM23 antibody (ab28304)
        Immunocytochemistry/ Immunofluorescence - Anti-ADAM23 antibody (ab28304)
        ICC/IF image of ab28304 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28304, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      References (1)

      Publishing research using ab28304? Please let us know so that we can cite the reference in this datasheet.

      ab28304 has been referenced in 1 publication.

      • McCracken L  et al. Improving the antibody-based evaluation of autoimmune encephalitis. Neurol Neuroimmunol Neuroinflamm 4:e404 (2017). PubMed: 29075658

      Customer reviews and Q&As

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