Anti-ADAM23 antibody (ab28304)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-ADAM23 antibody
See all ADAM23 primary antibodies -
Description
Rabbit polyclonal to ADAM23 -
Host species
Rabbit -
Specificity
ab28304 recognises the Propeptide domain of ADAM23. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to the Propeptide of Human ADAM23.
Read Abcam's proprietary immunogen policy (Peptide available as ab41222.)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab28304 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | ||
| WB |
Application notes
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 92 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 for chemiluminescent substrates. Predicted molecular weight: 92 kDa. EDTA/EGTA treatment of tissues or lysates is required to see latent zymogen. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
ADAM23 is a non-catalytic metalloprotease-like protein. It is highly expressed in the brain, primarily in the amygdala, caudate nucleus, hypothalamus, thalamus, cerebral cortex and occipital pole, and weakly expressed in the heart. 3 isoforms are produced by alternative splicing, alpha, beta and gamma. ADAM23 may play a role in cell-cell and cell-matrix interactions. -
Cellular localization
Single pass type I membrane protein. 3 isoforms produced by alternative splicing, gamma isoform is secreted. -
Database links
- Entrez Gene: 8745 Human
- Entrez Gene: 301460 Rat
- Omim: 603710 Human
- SwissProt: O75077 Human
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Alternative names
- A disintegrin and metalloproteinase domain 23 antibody
- ADAM metallopeptidase domain 23 antibody
- Disintegrin and metalloproteinase domain containing protein 23 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ADAM23 antibody (ab28304)ICC/IF image of ab28304 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab28304, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
This product has been referenced in:
- McCracken L et al. Improving the antibody-based evaluation of autoimmune encephalitis. Neurol Neuroimmunol Neuroinflamm 4:e404 (2017). Read more (PubMed: 29075658) »
