Anti-ADAM33 antibody (ab113740)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ADAM33 antibody -
Description
Rabbit polyclonal to ADAM33 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat
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Immunogen
Synthetic peptide corresponding to Mouse ADAM33 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab140321) -
Positive control
- This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following Mouse tissue lysates: Placenta; Lung; Spleen; Heart. This antibody gave a positive result in IHC in the following FFPE tissue: mouse normal placenta.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab113740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 87 kDa). | |
| IHC-P | Use a concentration of 5 µg/ml. |
Target
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Tissue specificity
Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins. -
Involvement in disease
Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 110751 Mouse
- Entrez Gene: 311425 Rat
- SwissProt: Q923W9 Mouse
- Unigene: 108550 Mouse
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Alternative names
- A disintegrin and metalloprotease 33 antibody
- A disintegrin and metalloproteinase domain 33 antibody
- ADA33_HUMAN antibody
see all
Images
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Western blot - Anti-ADAM33 antibody (ab113740)All lanes : Anti-ADAM33 antibody (ab113740) at 1 µg/ml
Lane 1 : Mouse Placenta Tissue Lysate
Lane 2 :Mouse lung normal tissue lysate - total protein (ab29297)
Lane 3 : Spleen (Mouse) Tissue Lysate
Lane 4 : Heart (Mouse) Tissue Lysate
Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 87 kDa
Observed band size: 108 kDa why is the actual band size different from the predicted?
Additional bands at: 28 kDa, 42 kDa, 68 kDa (possible cleavage fragment), 80 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
ADAM33 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of ADAM33 due to the presence of both a signal and pro peptide. The expression profile observed is consistent with what has been described in the literature (PMID: PMC2660367). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM33 antibody (ab113740)IHC image of ADAM33 staining in mouse normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113740, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Datasheets and documents
References
ab113740 has not yet been referenced specifically in any publications.
