• Product name
  • Description
    Rabbit polyclonal to ADAM33
  • Host species
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Mouse ADAM33.

  • Positive control
    • This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following Mouse tissue lysates: Placenta; Lung; Spleen; Heart. This antibody gave a positive result in IHC in the following FFPE tissue: mouse normal placenta.



Our Abpromise guarantee covers the use of ab113740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 87 kDa).
IHC-P Use a concentration of 5 µg/ml.


  • Tissue specificity
    Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
  • Involvement in disease
    Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloprotease 33 antibody
    • A disintegrin and metalloproteinase domain 33 antibody
    • ADA33_HUMAN antibody
    • ADAM 33 antibody
    • ADAM metallopeptidase domain 33 antibody
    • ADAM33 antibody
    • C20orf153 antibody
    • Disintegrin and metalloproteinase domain-containing protein 33 antibody
    • Disintegrin and reprolysin metalloproteinase family protein antibody
    • DJ964F7.1 antibody
    • DKFZp434K0521 antibody
    • FLJ35308 antibody
    • FLJ36751 antibody
    • Metalloprotease disintegrin antibody
    • MGC149823 antibody
    • MGC71889 antibody
    • PRO1891 antibody
    • UNQ873 antibody
    see all


  • All lanes : Anti-ADAM33 antibody (ab113740) at 1 µg/ml

    Lane 1 : Mouse Placenta Tissue Lysate
    Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
    Lane 3 : Spleen (Mouse) Tissue Lysate
    Lane 4 : Heart (Mouse) Tissue Lysate
    Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 87 kDa
    Observed band size: 108 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 28 kDa, 42 kDa, 68 kDa (possible cleavage fragment), 80 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 8 minutes

    ADAM33 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of ADAM33 due to the presence of both a signal and pro peptide. The expression profile observed is consistent with what has been described in the literature (PMID: PMC2660367).
  • IHC image of ADAM33 staining in mouse normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113740, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.


ab113740 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab113740.
Please use the links above to contact us or submit feedback about this product.


Sign up