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    adam33-antibody-ab113740.pdf

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Signal Transduction Cytoskeleton / ECM Extracellular Matrix ECM Enzymes MMP
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Anti-ADAM33 antibody (ab113740)

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Western blot - Anti-ADAM33 antibody (ab113740)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM33 antibody (ab113740)

Key features and details

  • Rabbit polyclonal to ADAM33
  • Suitable for: WB, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-ADAM33 antibody
  • Description

    Rabbit polyclonal to ADAM33
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide corresponding to Mouse ADAM33 aa 700 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab140321)

  • Positive control

    • This antibody gave a positive signal in NIH3T3 whole cell lysate as well as the following Mouse tissue lysates: Placenta; Lung; Spleen; Heart. This antibody gave a positive result in IHC in the following FFPE tissue: mouse normal placenta.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • MMP
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • ADAM Protein Family
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Metalloprotease
    • ADAMs

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

Our Abpromise guarantee covers the use of ab113740 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 108 kDa (predicted molecular weight: 87 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

  • Tissue specificity

    Expressed in all tissues, except liver, with high expression in placenta, lung, spleen and veins.
  • Involvement in disease

    Genetic variations in ADAM33 are associated with susceptibility to asthma (ASTHMA) [MIM:600807]. The most common chronic disease affecting children and young adults. It is a complex genetic disorder with a heterogeneous phenotype, largely attributed to the interactions among many genes and between these genes and the environment. It is characterized by recurrent attacks of paroxysmal dyspnea, with weezing due to spasmodic contraction of the bronchi.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Membrane.
  • Target information above from: UniProt accession Q9BZ11 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 110751 Mouse
    • Entrez Gene: 311425 Rat
    • SwissProt: Q923W9 Mouse
    • Unigene: 108550 Mouse
    • Alternative names

      • A disintegrin and metalloprotease 33 antibody
      • A disintegrin and metalloproteinase domain 33 antibody
      • ADA33_HUMAN antibody
      • ADAM 33 antibody
      • ADAM metallopeptidase domain 33 antibody
      • ADAM33 antibody
      • C20orf153 antibody
      • Disintegrin and metalloproteinase domain-containing protein 33 antibody
      • Disintegrin and reprolysin metalloproteinase family protein antibody
      • DJ964F7.1 antibody
      • DKFZp434K0521 antibody
      • FLJ35308 antibody
      • FLJ36751 antibody
      • Metalloprotease disintegrin antibody
      • MGC149823 antibody
      • MGC71889 antibody
      • PRO1891 antibody
      • UNQ873 antibody
      see all

    Images

    • Western blot - Anti-ADAM33 antibody (ab113740)
      Western blot - Anti-ADAM33 antibody (ab113740)
      All lanes : Anti-ADAM33 antibody (ab113740) at 1 µg/ml

      Lane 1 : Mouse Placenta Tissue Lysate
      Lane 2 : Mouse lung normal tissue lysate - total protein (ab29297)
      Lane 3 : Spleen (Mouse) Tissue Lysate
      Lane 4 : Heart (Mouse) Tissue Lysate
      Lane 5 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 87 kDa
      Observed band size: 108 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 28 kDa, 42 kDa, 68 kDa (possible cleavage fragment), 80 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes


      ADAM33 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted. The band observed at 68 kDa could potentially be a cleaved form of ADAM33 due to the presence of both a signal and pro peptide. The expression profile observed is consistent with what has been described in the literature (PMID: PMC2660367).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM33 antibody (ab113740)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAM33 antibody (ab113740)

      IHC image of ADAM33 staining in mouse normal placenta formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol B. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab113740, 5µg/ml, for 15 mins at room temperature. A Goat anti-Rabbit biotinylated secondary antibody was used to detect the primary, and visualized using an HRP conjugated ABC system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab113740? Please let us know so that we can cite the reference in this datasheet.

    ab113740 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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