Anti-ADAM9 antibody (ab186833)
Key features and details
- Rabbit polyclonal to ADAM9
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
-
Product name
Anti-ADAM9 antibody
See all ADAM9 primary antibodies -
Description
Rabbit polyclonal to ADAM9 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment corresponding to Human ADAM9 aa 475-685 (internal sequence).
Database link: Q13443 -
Positive control
- Mouse kidney and liver extracts.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186833 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB |
1/500 - 1/2000. Predicted molecular weight: 91 kDa.
|
|
IHC-P |
1/50 - 1/200.
|
Notes |
---|
ICC/IF
Use at an assay dependent concentration. |
WB
1/500 - 1/2000. Predicted molecular weight: 91 kDa. |
IHC-P
1/50 - 1/200. |
Target
-
Relevance
ADAM9 encodes disintegrin and metalloprotease (ADAM) domain 9, which is a member of the ADAM protein family. Members of this family are membrane- anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell- cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor. -
Cellular localization
Isoform 1: Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein. -
Database links
- Entrez Gene: 8754 Human
- Entrez Gene: 11502 Mouse
- Omim: 602713 Human
- SwissProt: Q13443 Human
- SwissProt: Q61072 Mouse
-
Alternative names
- A disintegrin and metalloproteinase domain 9 antibody
- ADAM 9 antibody
- ADAM metallopeptidase domain 9 antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling ADAM9 with ab186833 at 1/200. Magnification: 400x.
-
Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186833. Blue DAPI for nuclear staining.
-
All lanes : Anti-ADAM9 antibody (ab186833) at 1/500 dilution
Lane 1 : Mouse kidney extract
Lane 2 : Mouse liver extract
Predicted band size: 91 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (15)
ab186833 has been referenced in 15 publications.
- Xiao Y et al. Ubiquitin-specific peptidase 39 promotes human glioma cells migration and invasion by facilitating ADAM9 mRNA maturation. Mol Oncol 16:388-404 (2022). PubMed: 33811456
- Li D et al. IL-17A Promotes Epithelial ADAM9 Expression in Cigarette Smoke-Related COPD. Int J Chron Obstruct Pulmon Dis 17:2589-2602 (2022). PubMed: 36267325
- Goel K et al. Characterization of pulmonary vascular remodeling and MicroRNA-126-targets in COPD-pulmonary hypertension. Respir Res 23:349 (2022). PubMed: 36522710
- Kim DY et al. Involvement of Klotho, TNF-α and ADAMs in radiation-induced senescence of renal epithelial cells. Mol Med Rep 23:N/A (2021). PubMed: 33179086
- Liu B et al. mir-126-5p Promotes Cisplatin Sensitivity of Non-Small-Cell Lung Cancer by Inhibiting ADAM9. Biomed Res Int 2021:6622342 (2021). PubMed: 34055989