Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAM9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human ADAM9 aa 475-685 (internal sequence).
    Database link: Q13443

  • Positive control

    • Mouse kidney and liver extracts.

Properties

Applications

Our Abpromise guarantee covers the use of ab186833 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/2000. Predicted molecular weight: 91 kDa.
IHC-P 1/50 - 1/200.

Target

  • Relevance

    ADAM9 encodes disintegrin and metalloprotease (ADAM) domain 9, which is a member of the ADAM protein family. Members of this family are membrane- anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell- cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor.
  • Cellular localization

    Isoform 1: Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein.
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase domain 9 antibody
    • ADAM 9 antibody
    • ADAM metallopeptidase domain 9 antibody
    • Cellular disintegrin related protein antibody
    • Cone rod dystrophy 9 antibody
    • CORD9 antibody
    • Disintegrin and metalloproteinase domain 9 antibody
    • Disintegrin and metalloproteinase domain containing protein 9 antibody
    • KIAA0021 antibody
    • MCMP antibody
    • MDC 9 antibody
    • MDC9 antibody
    • Meltrin gamma antibody
    • Metalloprotease disintegrin cysteine rich protein 9 antibody
    • Mltng antibody
    • Myeloma cell metalloproteinase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver cancer tissue labelling ADAM9 with ab186833 at 1/200. Magnification: 400x.
  • Immunocytochemistry/Immunofluorescence analysis of U2OS cells using ab186833. Blue DAPI for nuclear staining.
  • All lanes : Anti-ADAM9 antibody (ab186833) at 1/500 dilution

    Lane 1 : Mouse kidney extract
    Lane 2 : Mouse liver extract

    Predicted band size: 91 kDa

References

This product has been referenced in:

  • Hua Y  et al. MicroRNA-126 inhibits proliferation and metastasis in prostate cancer via regulation of ADAM9. Oncol Lett 15:9051-9060 (2018). Read more (PubMed: 29805636) »
  • Yuan P  et al. KRAS/NF-?B/YY1/miR-489 Signaling Axis Controls Pancreatic Cancer Metastasis. Cancer Res 77:100-111 (2017). Read more (PubMed: 27793842) »
See all 4 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
Yes - 0.5% Triton TBS Ca/Azide
Specification
Skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Mr. Tyler Crowe

Verified customer

Submitted Mar 25 2019

Application
Flow Cytometry
Sample
Human Cell (Liver, Huh7.5)
Permeabilization
Yes - methanol
Gating Strategy
Huh7.5 cells were gated for SSC-FSC parameters
Specification
Liver, Huh7.5
Preparation
Cell harvesting/tissue preparation method: Rinse the cell layer with PBS and harvest the cells with 0.25% (w/v) Trypsin- EDTA solution.
Sample buffer: 0.25% (w/v) Trypsin- EDTA solution
Fixation
Methanol

Abcam user community

Verified customer

Submitted Jun 03 2015

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