Key features and details
- Rabbit polyclonal to ADAM9
- Suitable for: WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-ADAM9 antibody
See all ADAM9 primary antibodies
DescriptionRabbit polyclonal to ADAM9
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide within Human ADAM9 aa 400-450. The exact sequence is proprietary. NP_003807.1
Database link: Q13443
- WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab226459 was affinity purified using an epitope specific to ADAM9 immobilized on solid support.
Our Abpromise guarantee covers the use of ab226459 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/25000. Predicted molecular weight: 91 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
RelevanceADAM9 encodes disintegrin and metalloprotease (ADAM) domain 9, which is a member of the ADAM protein family. Members of this family are membrane- anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell- cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor.
Cellular localizationIsoform 1: Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein.
- A disintegrin and metalloproteinase domain 9 antibody
- ADAM 9 antibody
- ADAM metallopeptidase domain 9 antibody
All lanes : Anti-ADAM9 antibody (ab226459) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN lysis buffer
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using RIPA lysis buffer
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN lysis buffer
Lane 5 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate prepared using NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 91 kDa
Exposure time: 3 minutes
ADAM9 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226459 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226459 at 0.4 µg/ml.
Lane 1: ab226459 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226459 has not yet been referenced specifically in any publications.