Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAM9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human ADAM9 aa 400-450. The exact sequence is proprietary. NP_003807.1
    Database link: Q13443

  • Positive control

    • WB: HeLa, HEK-293T, Jurkat and TCMK-1 whole cell lysates. IP: HeLa whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226459 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/25000. Predicted molecular weight: 91 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Relevance

    ADAM9 encodes disintegrin and metalloprotease (ADAM) domain 9, which is a member of the ADAM protein family. Members of this family are membrane- anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biologic processes involving cell- cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The member encoded by this gene interacts with SH3 domain-containing proteins, binds mitotic arrest deficient 2 beta protein, and is also involved in TPA-induced ectodomain shedding of membrane-anchored heparin-binding EGF-like growth factor.
  • Cellular localization

    Isoform 1: Cell membrane; single-pass type I membrane protein. Isoform 2: Secreted protein.
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase domain 9 antibody
    • ADAM 9 antibody
    • ADAM metallopeptidase domain 9 antibody
    • Cellular disintegrin related protein antibody
    • Cone rod dystrophy 9 antibody
    • CORD9 antibody
    • Disintegrin and metalloproteinase domain 9 antibody
    • Disintegrin and metalloproteinase domain containing protein 9 antibody
    • KIAA0021 antibody
    • MCMP antibody
    • MDC 9 antibody
    • MDC9 antibody
    • Meltrin gamma antibody
    • Metalloprotease disintegrin cysteine rich protein 9 antibody
    • Mltng antibody
    • Myeloma cell metalloproteinase antibody
    see all

Images

  • All lanes : Anti-ADAM9 antibody (ab226459) at 0.04 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using NETN lysis buffer
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate prepared using RIPA lysis buffer
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate prepared using NETN lysis buffer
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate prepared using NETN lysis buffer
    Lane 5 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate prepared using NETN lysis buffer

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 91 kDa


    Exposure time: 3 minutes
  • ADAM9 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (prepared using RIPA buffer; 1 mg for IP, 20% of IP loaded) with ab226459 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226459 at 0.4 µg/ml.

    Lane 1: ab226459 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection: Chemiluminescence with exposure time of 3 seconds.

References

ab226459 has not yet been referenced specifically in any publications.

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