Recombinant Anti-ADAM9 antibody [EPR21924-164] (ab218242)
![Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-389422-anti-adam9-antibody-epr21924-164-western-blot-human-knockout-lysate.jpg "Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR21924-164] to ADAM9
- Suitable for: IP, WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-ADAM9 antibody [EPR21924-164]
See all ADAM9 primary antibodies -
Description
Rabbit monoclonal [EPR21924-164] to ADAM9 -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment within Human ADAM9 aa 400-700. The exact sequence is proprietary.
Database link: Q13443 -
Positive control
- WB: HepG2, HeLa, WI-38, MCF7, U-87 MG and A549 whole cell lysates. IP: HeLa whole cell lysate.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR21924-164 -
Isotype
IgG
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
Applications
Our Abpromise guarantee covers the use of ab218242 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IP | 1/30. | |
| WB | 1/1000. Detects a band of approximately 75, 100 kDa (predicted molecular weight: 91 kDa). |
Target
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Function
Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP. -
Tissue specificity
Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart. -
Involvement in disease
Cone-rod dystrophy 9 -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 EGF-like domain.
Contains 1 peptidase M12B domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 8754 Human
- Omim: 602713 Human
- SwissProt: Q13443 Human
- Unigene: 591852 Human
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Alternative names
- ADAM 9 antibody
- ADAM metallopeptidase domain 9 antibody
- Adam9 antibody
see all
Images
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Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : ADAM9 knockout HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 91 kDa
Observed band size: 100,75 kDa why is the actual band size different from the predicted?Lanes 1-3: Merged signal (red and green). Green - ab218242 observed at 100,75 kDa. Red - loading control ab8245 observed at 36 kDa.
ab218242 Anti-ADAM9 antibody [EPR21924-164] was shown to specifically react with ADAM9 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265484 (knockout cell lysate ab257814) was used. Wild-type and ADAM9 knockout samples were subjected to SDS-PAGE. ab218242 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-316600-anti-adam9-antibody-epr21924-164-western-blot.jpg)
Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution
Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
Lane 7 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 91 kDa
Observed band size: 100,75 kDa why is the actual band size different from the predicted?Blocking/diluting buffer and concentration: 5% NFDM/TBST
Exposure times:
Lanes 1-5: 3 minutes
Lane 6: 48 seconds
Lane 7: 114 secondsThe molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).
We recommend that customers avoid boiling their samples to prevent protein aggregation.
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![Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-316599-anti-adam9-antibody-epr21924-164-immunoprecipitation.jpg)
Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.
Lane 1: HeLa whole cell lysate 10μg (input).
Lane 2: ab218242 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.
The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).
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![Anti-ADAM9 antibody [EPR21924-164] (ab218242)](https://www.abcam.com/ps/products/218/ab218242/Images/ab218242-4-benefits-of-recombinant-antibodies.png)
Anti-ADAM9 antibody [EPR21924-164] (ab218242)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab218242 has not yet been referenced specifically in any publications.
