Recombinant
RabMAb

Recombinant Anti-ADAM9 antibody [EPR21924-164] (ab218242)

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Overview

  • Product name

    Anti-ADAM9 antibody [EPR21924-164]
    See all ADAM9 primary antibodies

  • Description

    Rabbit monoclonal [EPR21924-164] to ADAM9

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human ADAM9 aa 400-700. The exact sequence is proprietary.
    Database link: Q13443

  • Positive control

    • WB: HepG2, HeLa, WI-38, MCF7, U-87 MG and A549 whole cell lysates. IP: HeLa whole cell lysate.

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21924-164

  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab218242 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP 1/30.
WB 1/1000. Detects a band of approximately 75, 100 kDa (predicted molecular weight: 91 kDa).

Target

  • Function

    Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP.

  • Tissue specificity

    Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart.

  • Involvement in disease

    Cone-rod dystrophy 9

  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.

  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • ADAM 9 antibody
      • ADAM metallopeptidase domain 9 antibody
      • Adam9 antibody
      • ADAM9_HUMAN antibody
      • Cellular disintegrin-related protein antibody
      • Cone rod dystrophy 9 antibody
      • CORD9 antibody
      • Disintegrin and metalloproteinase domain-containing protein 9 antibody
      • MCMP antibody
      • MDC9 antibody
      • Meltrin-gamma antibody
      • Metalloprotease/disintegrin/cysteine-rich protein 9 antibody
      • Mltng antibody
      • Myeloma cell metalloproteinase antibody
      see all

    Images

    • Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      Western blot - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

      Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
      Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
      Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
      Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
      Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
      Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
      Lane 7 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

      Predicted band size: 91 kDa
      Observed band size: 100,75 kDa
      why is the actual band size different from the predicted?



      Blocking/diluting buffer and concentration:  5% NFDM/TBST 

      Exposure times:
      Lanes 1-5: 3 minutes
      Lane 6: 48 seconds
      Lane 7: 114 seconds

      The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).

      We recommend that customers avoid boiling their samples to prevent protein aggregation.

    • Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)
      Immunoprecipitation - Anti-ADAM9 antibody [EPR21924-164] (ab218242)

      ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

      Lane 1: HeLa whole cell lysate 10μg (input).
      Lane 2: ab218242 IP in HeLa whole cell lysate.
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.
      Exposure time: 3 minutes.

      The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.

      The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).

    References

    ab218242 has not yet been referenced specifically in any publications.

    Publishing research using ab218242? Please let us know so that we can cite the reference in this datasheet.

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