Recombinant
RabMAb

Recombinant Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free (ab238647)

Overview

  • Product name

    Anti-ADAM9 antibody [EPR21924-164] - BSA and Azide free
    See all ADAM9 primary antibodies
  • Description

    Rabbit monoclonal [EPR21924-164] to ADAM9 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment within Human ADAM9 aa 400-700. The exact sequence is proprietary.
    Database link: Q13443

  • Positive control

    • WB: HepG2, HeLa, WI-38, MCF7, U-87 MG and A549 whole cell lysate.
  • General notes

    Ab238647 is the carrier-free version of ab218242. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238647 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituent: PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR21924-164
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab238647 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 75, 100 kDa (predicted molecular weight: 91 kDa).
IP Use at an assay dependent concentration.

Target

  • Function

    Probable zinc protease. May mediate cell-cell or cell-matrix interactions. Isoform 2 displays alpha-secretase activity for APP.
  • Tissue specificity

    Widely expressed. Expressed in chondrocytes. Isoform 2 is highly expressed in liver and heart.
  • Involvement in disease

    Cone-rod dystrophy 9
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 EGF-like domain.
    Contains 1 peptidase M12B domain.
  • Cellular localization

    Secreted and Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • ADAM 9 antibody
    • ADAM metallopeptidase domain 9 antibody
    • Adam9 antibody
    • ADAM9_HUMAN antibody
    • Cellular disintegrin-related protein antibody
    • Cone rod dystrophy 9 antibody
    • CORD9 antibody
    • Disintegrin and metalloproteinase domain-containing protein 9 antibody
    • MCMP antibody
    • MDC9 antibody
    • Meltrin-gamma antibody
    • Metalloprotease/disintegrin/cysteine-rich protein 9 antibody
    • Mltng antibody
    • Myeloma cell metalloproteinase antibody
    see all

Images

  • ADAM9 was immunoprecipitated from 0.35 mg HeLa (human cervix adenocarcinoma cell) whole cell lysate with ab218242 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab218242 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/5000 dilution.

    Lane 1: HeLa whole cell lysate 10μg (input).
    Lane 2: ab218242 IP in HeLa whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab218242 in HeLa whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    The conformational epitope maybe changed after cleavage and this antibody has better affinity to the pro-form.

    The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218242).

  • All lanes : Anti-ADAM9 antibody [EPR21924-164] (ab218242) at 1/1000 dilution

    Lane 1 : HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate
    Lane 2 : HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate
    Lane 3 : WI-38 (human fetal lung fibroblast), whole cell lysate
    Lane 4 : MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate
    Lane 5 : U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate
    Lane 6 : A549 (human lung carcinoma epithelial cell), whole cell lysate
    Lane 7 : A431 (human epidermoid carcinoma epithelial cell), whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 91 kDa



    Blocking/diluting buffer and concentration:  5% NFDM/TBST 

    Exposure times:
    Lanes 1-5: 3 minutes
    Lane 6: 48 seconds
    Lane 7: 114 seconds

    The molecular mass observed is consistent with what has been described in the literature (PMID 29118335; PMID: 12767059; PMID: 20736367)

    We recommend that customers avoid boiling their samples to prevent protein aggregation.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab218242).

References

ab238647 has not yet been referenced specifically in any publications.

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