Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAMDEC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide derived from C terminal domain of mouse ADAMDEC1 protein.

Properties

Applications

Our Abpromise guarantee covers the use of ab67754 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500 - 1/5000. Predicted molecular weight: 53 kDa.
IHC-Fr Use at an assay dependent concentration.

Target

  • Function

    May play an important role in the control of the immune response and during pregnancy.
  • Tissue specificity

    Expressed highly in the small intestine and appendix, moderately in lymph node, mucosal lining of the colon, thymus, spleen and very weakly in the bone marrow. Predominantly expressed in dendritic cells (DC) of the germinal center. Weakly expressed in monocyte and highly expressed in macrophage. Absent in immature DC.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase domain like protein decysin 1 antibody
    • A disintegrin and metalloproteinase domain-like protein decysin-1 antibody
    • ADAM DEC1 antibody
    • ADAM like decysin 1 antibody
    • ADAM like protein decysin 1 antibody
    • ADAM-like protein decysin-1 antibody
    • ADAMDEC 1 antibody
    • Adamdec1 antibody
    • ADEC1_HUMAN antibody
    • Decysin antibody
    • Disintegrin protease antibody
    • M12.219 antibody
    see all

References

ab67754 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for your enquiry regarding ab67754 and ab101592. I am sorry to hear that you have been experiencing problems using these two products in the application that you wish. I can confirm that both antibodies have been tested for IHC-P and recognize murine proteins. We do not have any specific suggestions for antigen retrieval so the standard HIER (heat-induced epitope retrieval) should be fine, however optimization may be necessary for good and specific signal. Generally, we recommend using Tris/EDTA pH 9.0, sodium citrate pH 6.0 buffer and 3 minutes is as a starting point antigen retrieval time. Less than 3 minutes may leave the antigens under-retrieved, leading to weak staining. More than 3 minutes may leave them overretrieved, leading to non-specific background staining and also increasing the chances of sections dissociating from the slides. A control experiment is recommended beforehand, where slides of the same tissue section are retrieved for 1, 2, 3, 4 and 5 minutes before being immunohistochemically stained to evaluate optimum antigen retrieval time for the particular antibody being used. I could certainly take a look at your protocol if you wish and make some suggestions or tips which may improve the signal. If you are happy with this, could you complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response. Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

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