Anti-ADAMTS1 antibody (ab216977)
Key features and details
- Rabbit polyclonal to ADAMTS1
- Suitable for: IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS1 antibody
See all ADAMTS1 primary antibodies -
Description
Rabbit polyclonal to ADAMTS1 -
Host species
Rabbit -
Specificity
Contains 3 TSP type-1 domains. -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide within Mouse ADAMTS1 aa 900 to the C-terminus conjugated to keyhole limpet haemocyanin. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P97857 -
Positive control
- Human lung carcinoma tissue; Rat tongue tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216977 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Notes |
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IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200. |
Target
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 9510 Human
- Entrez Gene: 11504 Mouse
- Entrez Gene: 79252 Rat
- Omim: 605174 Human
- SwissProt: Q9UHI8 Human
- SwissProt: P97857 Mouse
- SwissProt: Q9WUQ1 Rat
- Unigene: 643357 Human
see all -
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
- ADAM-TS 1 antibody
- ADAM-TS1 antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Rat tongue tissue labeling ADAMTS1 with ab216977 at 1/200 dilution, followed by Goat Anti-rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution.
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Immunohistochemical analysis analysis of formalin-fixed and paraffin-embedded Human colon carcinoma tissue labeling ADAMTS1 with ab216977 at 1/200 dilution. Followed by Goat Anti-rabbit IgG, Cy3 conjugated secondary antibody at 1/200 dilution.
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded Human lung carcinoma tissue labeling ADAMTS1 with ab216977 at 1/200 dilution followed by conjugation to the secondary antibody and DAB staining.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab216977 has not yet been referenced specifically in any publications.