Product nameAnti-ADAMTS1 antibody
See all ADAMTS1 primary antibodies
DescriptionRabbit polyclonal to ADAMTS1
SpecificityAb39194 recognizes the zymogen of ADAMTS1, and the furin cleaved ADAMTS1, but not the shed form.
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human ADAMTS1 (C terminal). This antibody recognizes the last 28 amino acids of the protein.
Database link: Q9UHI8
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab39194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.|
FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 3 TSP type-1 domains.
DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
- ADAM-TS 1 antibody
- ADAM-TS1 antibody
ICC/IF image of ab39194 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39194, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab39194 staining ADAMTS1 in Human Placenta (Decidua) tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with a peroxidase block for 5 minutes at room temperature. Antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with primary antibody (1/50) in antibody diluent for 1 hour. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.
This product has been referenced in:
- Someya A et al. Glucosamine Downregulates the IL-1ß-Induced Expression of Proinflammatory Cytokine Genes in Human Synovial MH7A Cells by O-GlcNAc Modification-Dependent and -Independent Mechanisms. PLoS One 11:e0165158 (2016). WB ; Human . Read more (PubMed: 27776166) »
- Chen J et al. Downregulation of A disintegrin and metallopeptidase with thrombospondin motif type 1 by DNA hypermethylation in human gastric cancer. Mol Med Rep 12:2487-94 (2015). ICC/IF ; Human . Read more (PubMed: 25936341) »