Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAMTS1
  • Host species

    Rabbit
  • Specificity

    Ab39194 recognizes the zymogen of ADAMTS1, and the furin cleaved ADAMTS1, but not the shed form.
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human ADAMTS1 (C terminal). This antibody recognizes the last 28 amino acids of the protein.
    Database link: Q9UHI8

Properties

Applications

Our Abpromise guarantee covers the use of ab39194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
WB Use at an assay dependent concentration. Predicted molecular weight: 105 kDa.

Target

  • Function

    Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover (By similarity). Has angiogenic inhibitor activity. Active metalloprotease, which may be associated with various inflammatory processes as well as development of cancer cachexia. May play a critical role in follicular rupture.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 3 TSP type-1 domains.
  • Domain

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase with thrombospondin motifs 1 antibody
    • ADAM-TS 1 antibody
    • ADAM-TS1 antibody
    • ADAMTS 1 antibody
    • ADAMTS antibody
    • ADAMTS-1 antibody
    • Adamts1 antibody
    • ATS1_HUMAN antibody
    • C3 C5 antibody
    • KIAA1346, antibody
    • METH 1 antibody
    • METH-1 antibody
    see all

Images

  • ICC/IF image of ab39194 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39194, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab39194 staining ADAMTS1 in Human Placenta (Decidua) tissue sections by IHC-P (Paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with a peroxidase block for 5 minutes at room temperature. Antigen retrieval was by heat mediation in sodium citrate. Samples were incubated with primary antibody (1/50) in antibody diluent for 1 hour. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Dosh RH  et al. Long-term in vitro 3D hydrogel co-culture model of inflammatory bowel disease. Sci Rep 9:1812 (2019). Read more (PubMed: 30755679) »
  • Someya A  et al. Glucosamine Downregulates the IL-1ß-Induced Expression of Proinflammatory Cytokine Genes in Human Synovial MH7A Cells by O-GlcNAc Modification-Dependent and -Independent Mechanisms. PLoS One 11:e0165158 (2016). WB ; Human . Read more (PubMed: 27776166) »
See all 6 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (Heart)
Gel Running Conditions
Non-reduced Denaturing (4-12%)
Loading amount
10 µg
Specification
Heart
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Oct 25 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta (Decidua))
Specification
Placenta (Decidua)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate
Blocking step
Dako Peroxidase block-> Dako Protein block serum-free ready to use as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: RT°C

Ms. Hyangsin Lee

Verified customer

Submitted Feb 23 2010

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