Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAMTS10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Pig, Monkey
  • Immunogen

    Synthetic peptide corresponding to Human ADAMTS10 (internal sequence) conjugated to keyhole limpet haemocyanin.
    Database link: Q9H324

  • Positive control

    • WB: HeLa, PC-12 and RAW 264.7 whole cell lysates. IHC-P: Human heart vessel tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab230902 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 121 kDa.
IHC-P 1/100.

Target

  • Function

    Metalloprotease that participate in microfibrils assembly. Microfibrils are extracellular matrix components occurring independently or along with elastin in the formation of elastic tissues.
  • Tissue specificity

    Widely expressed in adult tissues.
  • Involvement in disease

    Defects in ADAMTS10 are the cause of Weill-Marchesani syndrome 1 (WMS1) [MIM:277600]. WMS1 is a rare connective tissue disorder characterized by short stature, brachydactyly, joint stiffness, and eye abnormalities including microspherophakia, ectopia lentis, severe myopia and glaucoma.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 PLAC domain.
    Contains 5 TSP type-1 domains.
  • Domain

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
  • Post-translational
    modifications

    Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase with thrombospondin motifs 10 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 10 antibody
    • A disintegrin-like and metallopeptidase with thrombospondin type 1 motif, 10 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 10 antibody
    • ADAM TS10 antibody
    • ADAM-TS 10 antibody
    • ADAM-TS10 antibody
    • ADAMTS-10 antibody
    • Adamts10 antibody
    • ATS10_HUMAN antibody
    • EC 3.4.24 antibody
    • WMS antibody
    • WMS1 antibody
    • zinc metalloendopeptidase antibody
    see all

Images

  • All lanes : Anti-ADAMTS10 antibody (ab230902) at 1/500 dilution

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 2 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Predicted band size: 121 kDa

  • Formalin-fixed, paraffin-embedded human heart vessel tissue stained for ADAMTS10 using ab230902 at 1/100 dilution in immunohistochemical analysis.

References

ab230902 has not yet been referenced specifically in any publications.

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