Key features and details
- Rabbit polyclonal to ADAMTS12
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45041 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
RelevanceThis protein is a disintegrin and metalloproteinase with thrombospondin motifs 12 (ADAMTS12), which is a member of the ADAMTS protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin like domain, and a thrombospondin type 1 (TS 1) motif. Individual members of this family differ in the number of C terminal TS 1 motifs, and some have unique C terminal domains. The enzyme encoded by this gene contains 8 TS 1 motifs. It may play roles in pulmonary cells during fetal development or in tumor processes through its proteolytic activity or as a molecule potentially involved in regulation of cell adhesion.
- A disintegrin and metalloproteinase with thrombospondin motifs 12 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 12 antibody
- ADAM metallopeptidase with thrombospondin type 1 motif 12 antibody
- ADAM TS 12 antibody
All lanes : Anti-ADAMTS12 antibody (ab45041) at 1/1000 dilution
Lane 1 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TPA)
Lane 2 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with IL1-alpha)
Lane 3 : Cell media from mouse squamous cell carcinoma KLN 205 cells (treated with TNF-alpha)
Predicted band size: 178 kDa
Full-length ADAMTS12 has been detected at 178 kDa by WB. However, study looking at ADAM-TS 12 expression in COS-7 cells (Gal et al. (2001) J. Biol. Chem. 276: 17932), has detected an 83 kD COOH-terminal cleavage product.It is possible that the post-translation processing in COS-7 cells is different than that of other cells, that the epitope tag is cleaved during processing, or that the levels of the 83 kD product in other cells were below detection.
ab45041 has been referenced in 1 publication.
- Acar-Perk B et al. The t(14,15) in mouse strain CBA/CaH-T(14;15)6Ca/J causes a break in the ADAMTS12 gene. Comp Med 60:118-22 (2010). WB ; Mouse . PubMed: 20412686