Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAMTS13
  • Host species

    Rabbit
  • Specificity

    This antibody reacts specifically with human 154 kDa ADAMTS13 protein
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide derived from the N-terminal domain of ADAMTS13 protein.

  • Positive control

    • This antibody gave a positive signal in mouse liver tissue lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab71550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 154 kDa (predicted molecular weight: 154 kDa).

Target

  • Function

    Cleaves the vWF multimers in plasma into smaller forms.
  • Tissue specificity

    Plasma. Expressed primarily in liver.
  • Involvement in disease

    Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.
  • Sequence similarities

    Contains 2 CUB domains.
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 8 TSP type-1 domains.
  • Domain

    The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
    The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.
  • Post-translational
    modifications

    May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
    The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody
    • ADAM TS antibody
    • ADAM-TS 13 antibody
    • ADAM-TS13 antibody
    • ADAMTS 13 antibody
    • ADAMTS-13 antibody
    • ADAMTS13 antibody
    • ADAMTS13 protein antibody
    • ATS13_HUMAN antibody
    • C9orf8 antibody
    • TTP antibody
    • Von Willebrand factor cleaving protease antibody
    • von Willebrand factor-cleaving protease antibody
    • vWF cleaving protease antibody
    • vWF CP antibody
    • vWF-cleaving protease antibody
    • vWF-CP antibody
    • vWFCP antibody
    see all

Images

  • Anti-ADAMTS13 antibody (ab71550) at 1/500 dilution + Liver (Mouse) Tissue Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 154 kDa
    Observed band size: 154 kDa
    Additional bands at: 37 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 20 minutes

References

This product has been referenced in:

  • Bartoli CR  et al. Insights into the mechanism(s) of von Willebrand factor degradation during mechanical circulatory support. J Thorac Cardiovasc Surg N/A:N/A (2013). WB . Read more (PubMed: 24139617) »
  • Pos W  et al. An autoantibody epitope comprising residues R660, Y661, and Y665 in the ADAMTS13 spacer domain identifies a binding site for the A2 domain of VWF. Blood 115:1640-9 (2010). WB ; Human . Read more (PubMed: 20032502) »
See all 2 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (hepatocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

Submitted Sep 10 2015

Answer

Laut unseren Unterlagen hatten Sie Probleme mit ab71550 Anti-ADAMTS13 antibody und haben unseren technischen Dienst deswegen kontaktiert.

Ich sehe, dass wir noch auf einige Angaben hinsichtlich Ihrer Bestellung warten, die uns helfen werden Ihr Problem zu lösen. Falls Sie diese Informationen schon an uns geschickt haben, sieht es so aus, als ob diese nicht bei uns angekommen sind und ich möchte Sie daher bitten, diese Informationen nochmals an mich zu senden. Ich möchte Sie ausserdem für diesen Umstand um Entschuldigung bitten.

Falls Sie das Problem selbst gelöst haben, möchte ich Sie bitten, mir dieses kurz mitzuteilen, damit ich Ihre Anfrage aus dem System löschen kann.

Ich wünsche Ihnen viel Erfolg bei Ihrem Projekt und würde mich freuen von Ihnen zu hören, wie Sie vorankommen. Bitte zögern Sie nicht sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Answer

Thank you for your enquiry.

I can confirm that ab71550 antibody is sold as whole antiserum. Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant contains a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to vary between 1 - 10 mg/ml.

I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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