Recombinant Anti-ADAMTS13 antibody [EPR6132] (ab177940)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6132] to ADAMTS13
- Suitable for: WB, Flow Cyt
- Reacts with: Human
Overview
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Product name
Anti-ADAMTS13 antibody [EPR6132]
See all ADAMTS13 primary antibodies -
Description
Rabbit monoclonal [EPR6132] to ADAMTS13 -
Host species
Rabbit -
Tested Applications & Species
Application Species Flow Cyt HumanWB Human -
Immunogen
Synthetic peptide within Human ADAMTS13 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
Database link: Q76LX8 -
Positive control
- Human fetal liver, A549 and HepG2 whole cell lysate (ab7900); A549 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6132 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab177940 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
Application | Species |
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Flow Cyt |
Human
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ICC |
Human
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WB |
Human
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Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa).
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Flow Cyt |
1/100 - 1/500.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa). |
Flow Cyt
1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Cleaves the vWF multimers in plasma into smaller forms. -
Tissue specificity
Plasma. Expressed primarily in liver. -
Involvement in disease
Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever. -
Sequence similarities
Contains 2 CUB domains.
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 8 TSP type-1 domains. -
Domain
The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction. -
Post-translational
modificationsMay contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
The precursor is processed by a furin endopeptidase which cleaves off the pro-domain. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 11093 Human
- Omim: 604134 Human
- SwissProt: Q76LX8 Human
- Unigene: 131433 Human
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
see all
Images
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All lanes : Anti-ADAMTS13 antibody [EPR6132] (ab177940) at 1/1000 dilution
Lane 1 : Human fetal liver lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 154 kDa -
Flow cytometric analysis of permeabilized A549 cells labeling ADAMTS13 with ab177940 at 1/100 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab177940 has been referenced in 1 publication.
- Liu-Chen S et al. mRNA treatment produces sustained expression of enzymatically active human ADAMTS13 in mice. Sci Rep 8:7859 (2018). PubMed: 29777164