RecombinantRabMAb

Recombinant Anti-ADAMTS13 antibody [EPR6132] (ab177940)

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Western blot - Anti-ADAMTS13 antibody [EPR6132] (ab177940)
  • Flow Cytometry - Anti-ADAMTS13 antibody [EPR6132] (ab177940)
  • Anti-ADAMTS13 antibody [EPR6132] (ab177940)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR6132] to ADAMTS13
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-ADAMTS13 antibody [EPR6132]
    See all ADAMTS13 primary antibodies

  • Description

    Rabbit monoclonal [EPR6132] to ADAMTS13

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human ADAMTS13 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q76LX8

  • Positive control

    • Human fetal liver, A549 and HepG2 whole cell lysate (ab7900); A549 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab177940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
Notes
WB
1/1000 - 1/5000. Detects a band of approximately 190 kDa (predicted molecular weight: 154 kDa).
Flow Cyt
1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
  • Application notes
    Is unsuitable for ICC,IHC-P or IP.

    • Target

    • Function

      Cleaves the vWF multimers in plasma into smaller forms.

    • Tissue specificity

      Plasma. Expressed primarily in liver.

    • Involvement in disease

      Defects in ADAMTS13 are the cause of thrombotic thrombocytopenic purpura congenital (TTP) [MIM:274150]; also known as Upshaw-Schulman syndrome (USS). A hematologic disease characterized by hemolytic anemia with fragmentation of erythrocytes, thrombocytopenia, diffuse and non-focal neurologic findings, decreased renal function and fever.

    • Sequence similarities

      Contains 2 CUB domains.
      Contains 1 disintegrin domain.
      Contains 1 peptidase M12B domain.
      Contains 8 TSP type-1 domains.

    • Domain

      The pro-domain is not required for folding or secretion and does not perform the common function of maintening enzyme latency.
      The spacer domain is necessary to recognize and cleave vWF. The C-terminal TSP type-1 and CUB domains may modulate this interaction.

    • Post-translational
      modifications

      May contain a C-mannosylation site and O-fucosylation sites in the TSP type-1 domains.
      The precursor is processed by a furin endopeptidase which cleaves off the pro-domain.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • A disintegrin and metalloproteinase with thrombospondin motifs 13 antibody
        • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 13 antibody
        • A disintegrin like and metalloprotease with thrombospondin type 1 motif 13 antibody
        • ADAM metallopeptidase with thrombospondin type 1 motif 13 antibody
        • ADAM TS antibody
        • ADAM-TS 13 antibody
        • ADAM-TS13 antibody
        • ADAMTS 13 antibody
        • ADAMTS-13 antibody
        • ADAMTS13 antibody
        • ADAMTS13 protein antibody
        • ATS13_HUMAN antibody
        • C9orf8 antibody
        • TTP antibody
        • Von Willebrand factor cleaving protease antibody
        • von Willebrand factor-cleaving protease antibody
        • vWF cleaving protease antibody
        • vWF CP antibody
        • vWF-cleaving protease antibody
        • vWF-CP antibody
        • vWFCP antibody
        see all

      Images

      • Western blot - Anti-ADAMTS13 antibody [EPR6132] (ab177940)
        Western blot - Anti-ADAMTS13 antibody [EPR6132] (ab177940)
        All lanes : Anti-ADAMTS13 antibody [EPR6132] (ab177940) at 1/1000 dilution

        Lane 1 : Human fetal liver lysate
        Lane 2 : A549 cell lysate
        Lane 3 : HepG2 lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 154 kDa

      • Flow Cytometry - Anti-ADAMTS13 antibody [EPR6132] (ab177940)
        Flow Cytometry - Anti-ADAMTS13 antibody [EPR6132] (ab177940)

        Flow cytometric analysis of permeabilized A549 cells labeling ADAMTS13 with ab177940 at 1/100 dilution.

      • Anti-ADAMTS13 antibody [EPR6132] (ab177940)
        Anti-ADAMTS13 antibody [EPR6132] (ab177940)

      References (1)

      Publishing research using ab177940? Please let us know so that we can cite the reference in this datasheet.

      ab177940 has been referenced in 1 publication.

      • Liu-Chen S  et al. mRNA treatment produces sustained expression of enzymatically active human ADAMTS13 in mice. Sci Rep 8:7859 (2018). PubMed: 29777164

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