Overview

  • Product name
  • Description
    Rabbit polyclonal to ADAMTS15
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human, Rhesus monkey
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    HSTNRLTLAVAW

    , corresponding to amino acids 592-603 of Human ADAMTS15

  • Positive control
    • 293EBNA cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab28516 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 103 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 21791558

Target

  • Tissue specificity
    Expressed in fetal liver and kidney, but not in any of the adult tissues examined.
  • Sequence similarities
    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 3 TSP type-1 domains.
  • Domain
    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications
    The precursor is cleaved by a furin endopeptidase.
    Glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs, and N-glycosylated. These other glycosylations can also facilitate secretion.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • A disintegrin and metalloproteinase with thrombospondin motifs 15 antibody
    • A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 antibody
    • A disintegrin like and metalloprotease reprolysin type with thrombospondin type 1 motif 15 preproprotein antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 15 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 15 preproprotein antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 15 antibody
    • ADAM TS 15 antibody
    • ADAM TS15 antibody
    • ADAM-TS 15 antibody
    • ADAM-TS15 antibody
    • ADAMTS 15 antibody
    • ADAMTS-15 antibody
    • ADAMTS15 antibody
    • ATS15_HUMAN antibody
    • MGC126403 antibody
    see all

Images

  • Anti-ADAMTS15 antibody (ab28516) at 1/1000 dilution + Supernatant of 293 EBNA cells overexpressing ADAMTS15. at 20 µl

    Predicted band size: 103 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    The image shows an overexpressed fragment of ADAMTS15, not the full-length protein.

References

This product has been referenced in:
  • Demircan K  et al. ADAMTS4 and ADAMTS5 Knockout Mice Are Protected from Versican but Not Aggrecan or Brevican Proteolysis during Spinal Cord Injury. Biomed Res Int 2014:693746 (2014). WB ; Mouse . Read more (PubMed: 25101296) »
  • Peluffo MC  et al. Systematic analysis of protease gene expression in the rhesus macaque ovulatory follicle: metalloproteinase involvement in follicle rupture. Endocrinology 152:3963-74 (2011). IHC-P ; Rhesus monkey . Read more (PubMed: 21791558) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us with your question and for your patience while I have been in touch with the lab regarding your enquiry.

I've heard back from the lab, and the antibody was actually tested on a recombinantly expressed fragment of the ADAMTS15 protein in 293 EBNA cells.This fragment is what is seen in the Western Blot on our website, and as it is not the full length protein it runs much lower on the blot. However, the antibody should recognize full size ADAMTS-15 at the expected 103 kDa as well.

I hope this information will be useful, but please let me know if you have further questions or if there is anything else that we can do for you and I'll be happy to help.

Read More
Application
Western blot
Sample
Human Cell lysate - whole cell (MDAMB231 breast cancer cells)
Loading amount
30 µg
Specification
MDAMB231 breast cancer cells
Gel Running Conditions
Non-reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Apr 05 2010

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