Anti-ADAMTS16 antibody (ab45048)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-ADAMTS16 antibody
See all ADAMTS16 primary antibodies -
DescriptionRabbit polyclonal to ADAMTS16
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Host speciesRabbit
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide based on the propeptide region of human ADAMTS-16.
(Peptide available as ab45246.)
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General notes
We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS16 antibodies.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4 -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab45048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
WB: 1/1000 - 1/5000. Predicted molecular weight: 136 kDa. The recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceADAMTS16 is a member of the ADAMs family of proteinases with 4 thrombospondin-like domains. ADAMTS16 is closest in homology to ADAMTS18, sharing 57% overall identity. ADAMTS16 is expressed in fetal human lung and kidney, and in adult brain and ovary as well as in osteoarthritic cartilage, prostate and breast tissue. ADAMTS16 contains a prohormone-convertase cleavage site; the predicted PC cleavage site is the RRRR87 sequence in the 1224 and 1072 amino acid forms. This catalytic site is typical of the metalloproteinase catalytic domains, with an HExxHxxxxxH sequence. Several splice variants of ADAMTS16 have been reported.
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Cellular localizationSecreted
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Database links
- Entrez Gene: 170690 Human
- Entrez Gene: 271127 Mouse
- Omim: 607510 Human
- SwissProt: Q8TE57 Human
- SwissProt: Q69Z28 Mouse
- Unigene: 387861 Human
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Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 16 antibody
- ADAMTS 16 antibody
- ADAMTS16 antibody
- EC 3.4.24. antibody
Images
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Western blot - Anti-ADAMTS16 antibody (ab45048)All lanes : Anti-ADAMTS16 antibody (ab45048) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with SF)
Lane 2 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with TPA)
Lane 3 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with IL1-alpha)
Lane 4 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with RA)
Predicted band size: 136 kDa
Several different splice variants of ADAMTS-16 have been reported: 151.38 kDa, 136.2 kDa and 119.5 kDa.Two shorter versions of 62kDa and 56kDa are also reported, lacking the MP domain.
Protocols
References
This product has been referenced in:
- Jacobi CL et al. Transcriptional regulation by the Wilms tumor protein, Wt1, suggests a role of the metalloproteinase Adamts16 in murine genitourinary development. J Biol Chem 288:18811-24 (2013). Read more (PubMed: 23661704) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"