Anti-ADAMTS16 antibody (ab45048)

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Western blot - Anti-ADAMTS16 antibody (ab45048)

    Key features and details

    • Rabbit polyclonal to ADAMTS16
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    Overview

    • Product name

      Anti-ADAMTS16 antibody
      See all ADAMTS16 primary antibodies

    • Description

      Rabbit polyclonal to ADAMTS16

    • Host species

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data

    • Immunogen

      Synthetic peptide based on the propeptide region of human ADAMTS-16.

      Read Abcam's proprietary immunogen policy (Peptide available as ab45246.)

    • General notes

      We have a range of domain specific antibodies for this target. For a full list please see all ADAMTS16 antibodies.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid

    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

    • Storage buffer

      pH: 7.40
      Preservative: 0.05% Sodium azide
      Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
    • Concentration information loading...

    • Purity

      Immunogen affinity purified

    • Clonality

      Polyclonal

    • Isotype

      IgG

    • Research areas

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab45048 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Mouse
    Application Abreviews Notes
    WB
    Notes
  • Application notes
    WB: 1/1000 - 1/5000. Predicted molecular weight: 136 kDa. The recommended starting concentration for Western blots is 1:1,000 when using colorimetric substrates such as BCIP/NBT, and 1:5,000 for chemiluminescent substrates. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Relevance

      ADAMTS16 is a member of the ADAMs family of proteinases with 4 thrombospondin-like domains. ADAMTS16 is closest in homology to ADAMTS18, sharing 57% overall identity. ADAMTS16 is expressed in fetal human lung and kidney, and in adult brain and ovary as well as in osteoarthritic cartilage, prostate and breast tissue. ADAMTS16 contains a prohormone-convertase cleavage site; the predicted PC cleavage site is the RRRR87 sequence in the 1224 and 1072 amino acid forms. This catalytic site is typical of the metalloproteinase catalytic domains, with an HExxHxxxxxH sequence. Several splice variants of ADAMTS16 have been reported.

    • Cellular localization

      Secreted

    • Database links

      • Alternative names

        • A disintegrin and metalloproteinase with thrombospondin motifs 16 antibody
        • ADAMTS 16 antibody
        • ADAMTS16 antibody
        • EC 3.4.24. antibody

      Images

      • Western blot - Anti-ADAMTS16 antibody (ab45048)
        Western blot - Anti-ADAMTS16 antibody (ab45048)
        All lanes : Anti-ADAMTS16 antibody (ab45048) at 1/1000 dilution

        Lane 1 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with SF)

        Lane 2 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with TPA)

        Lane 3 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with IL1-alpha)

        Lane 4 : Whole cell lysate prepared from mouse lung mesenchymal MLg cells (treated with RA)


        Predicted band size: 136 kDa



        Several different splice variants of ADAMTS-16 have been reported: 151.38 kDa, 136.2 kDa and 119.5 kDa.Two shorter versions of 62kDa and 56kDa are also reported, lacking the MP domain.

      References (3)

      Publishing research using ab45048? Please let us know so that we can cite the reference in this datasheet.

      ab45048 has been referenced in 3 publications.

      • Mani S  et al. Epigenetic changes in preterm birth placenta suggest a role for ADAMTS genes in spontaneous preterm birth. Hum Mol Genet 28:84-95 (2019). PubMed: 30239759
      • Cao M  et al. Metalloproteinase Adamts16 Is Required for Proper Closure of the Optic Fissure. Invest Ophthalmol Vis Sci 59:1167-1177 (2018). PubMed: 29625437
      • Jacobi CL  et al. Transcriptional regulation by the Wilms tumor protein, Wt1, suggests a role of the metalloproteinase Adamts16 in murine genitourinary development. J Biol Chem 288:18811-24 (2013). PubMed: 23661704

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