Key features and details
- Rabbit polyclonal to ADAMTS4
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADAMTS4 antibody
See all ADAMTS4 primary antibodies
DescriptionRabbit polyclonal to ADAMTS4
SpecificityBLAST analysis of the peptide immunogen showed no homology with other Human proteins.
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Horse, Dog, Pig, Orangutan, Bat
Synthetic peptide within Human ADAMTS4. The exact sequence is proprietary. 16 amino acid peptide from the Metalloprotease domain.
Database link: O75173
- IHC-P; Human skin melanoma, Human cartilage and Human kidney tissue.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.099% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab219548 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
Tissue specificityExpressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 TSP type-1 domain.
DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
- A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human skin melanoma tissue labeling ADAMTS4 with ab219548 at 4 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cartilage tissue labeling ADAMTS4 with ab219548 at 4 µg/ml.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling ADAMTS4 with ab219548 at 4 µg/ml.
ab219548 has not yet been referenced specifically in any publications.