Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAMTS4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide from the C-terminal of Human ADAMTS4 (NM_005099).

  • Positive control

    • IHC-P: Human brain tissue. WB: Human fetal brain lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab84792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/500. Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
IHC-P 1/100 - 1/500.

Target

  • Function

    Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. Could also be a critical factor in the exacerbation of neurodegeneration in Alzheimer disease. Cleaves aggrecan at the '392-Glu-
    -Ala-393' site.
  • Tissue specificity

    Expressed in brain, lung and heart. Expressed at very low level in placenta and skeletal muscles.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 TSP type-1 domain.
  • Domain

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloproteinase with thrombospondin motifs 4 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 4 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 4 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 4 antibody
    • ADAM TS 4 antibody
    • ADAM TS4 antibody
    • ADAM-TS 4 antibody
    • ADAM-TS4 antibody
    • ADAMTS 2 antibody
    • ADAMTS 4 antibody
    • ADAMTS-4 antibody
    • ADAMTS2 antibody
    • ADAMTS4 antibody
    • ADAMTS4 protein antibody
    • ADMP 1 antibody
    • ADMP-1 antibody
    • ADMP1 antibody
    • Aggrecanase 1 antibody
    • Aggrecanase-1 antibody
    • Aggrecanase1 antibody
    • ATS4_HUMAN antibody
    • KIAA0688 antibody
    see all

Images

  • Anti-ADAMTS4 antibody (ab84792) at 1/1000 dilution + Human fetal brain lysates

    Predicted band size: 90 kDa
    Observed band size: 90 kDa

  • ab84792 at a 1/200 dilution staining ADAMTS4 in Human brain tissue.

References

This product has been referenced in:

  • Chen J  et al. Promotion of Tumor Growth by ADAMTS4 in Colorectal Cancer: Focused on Macrophages. Cell Physiol Biochem 46:1693-1703 (2018). Read more (PubMed: 29694979) »
  • Wu J  et al. Wnt-ß-catenin signaling pathway inhibition by sclerostin may protect against degradation in healthy but not osteoarthritic cartilage. Mol Med Rep 15:2423-2432 (2017). WB ; Human . Read more (PubMed: 28259981) »
See all 7 Publications for this product

Customer reviews and Q&As

Answer

Thank you for contacting us.

We guarantee many of the ADAMTS4 antibodies in our catalog to work in Western Blot on human derived samples. While I admit that I am not an expert on this target I assume that because you are looking to test the conditioned medium as well as a protein lysate that you would prefer to use a product that would detect the mature form of ADAMST4. According to the Uniprot/SwissProt data for ADAMST4 (http://www.uniprot.org/uniprot/O75173) the propeptide region of this protein ends at aa213, therefore any product raised against a region beyond should be acceptable for your studies.

We have 4 such products, ab111905, ab28285, ab82300 and ab84792. These are all rabbit polyclonal antibodies. As we do receive some of the products on our catalog from outside laboratories and other sources we have not tested these products against one another I would not be able to tell you if one of these was better than another. However, I do recommend choosing products with more characterization data (publications, Abreviews, more tested applications). Another consideration in this group is that ab111905 is actually against an area near to the N terminus of the mature protein, while the others are against the C-terminus. After review of all this I would recommend one of the following 2 products; ab84792 or ab82300.

Please remember, these products are covered by our Abpromise guarantee. We provide scientific support, replacement or refund should this product not perform as indicated on the datasheet. More information on our Abpromise may be found at the following link:

https://www.abcam.com/index.html?pageconfig=abpromise

We also provide a number of Western blot reagents and kits including our Optiblot ECL, ECL Max and ELC Ultra Detect kitswhich our enhanced chemiluminescent substrates specially developed for film imaging. These Optiblot ECL Detect Kits produce strong signal with very low background for high sensitivity. These products, as well as all of our Optiblot line, are also featured in our August promotion for 25% off when using the codeNEW-T7193before 31 Aug 2012. More information about our ECL kits, our Optiblot range and our August promotions may be found at the links below:

Optiblot ECL Detect Kit (23pg-187ng):https://www.abcam.com/Optiblot-ECL-Detect-Kit-23pg-187ng-ab133406.html

Optiblot ECL Max Detect Kit (4.6pg-4.7ng):https://www.abcam.com/Optiblot-ECL-Max-Detect-Kit-46pg-47ng-ab133408.html

Optiblot ECL Ultra Detect Kit (1.2pg-2ng):https://www.abcam.com/Optiblot-ECL-Ultra-Detect-Kit-12pg-2ng-ab133409.html

Our entire Optiblot range:https://www.abcam.com/index.html?pageconfig=resource&rid=14709

Our August Promotions;https://www.abcam.com/index.html?pageconfig=resource&rid=15134&source=pagetrap&viapagetrap=new25

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up