Key features and details
- Rabbit polyclonal to ADAMTS5
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ADAMTS5 antibody
See all ADAMTS5 primary antibodies
DescriptionRabbit polyclonal to ADAMTS5
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- Human tonsil tissue.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS
(without Mg2+ and Ca2+)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab182795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/10 - 1/50.|
|WB||1/500 - 1/2000. Predicted molecular weight: 102 kDa.|
FunctionCleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period.
Tissue specificityExpressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains.
DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling ADAMTS5 with ab182795 at 1/10. Image on the right is treated with the synthetic peptide.
Anti-ADAMTS5 antibody (ab182795) at 1/650 dilution + Human fetal brain tissue at 40 µg
Goat anti Rabbit IgG at 1/3000 dilution
Predicted band size: 102 kDa
Exposure time: 3 seconds
ab182795 has not yet been referenced specifically in any publications.