Anti-ADAMTS5 antibody (ab182795)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-ADAMTS5 antibody
See all ADAMTS5 primary antibodies -
Description
Rabbit polyclonal to ADAMTS5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ADAMTS5 aa 529-541.
Sequence:CLTKKLPAVEGTP
Database link: Q9UNA0 -
Positive control
- Human tonsil tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS
(without Mg2+ and Ca2+) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab182795 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/10 - 1/50. | |
| WB | 1/500 - 1/2000. Predicted molecular weight: 102 kDa. |
Target
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. -
Tissue specificity
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 11096 Human
- Entrez Gene: 23794 Mouse
- Entrez Gene: 304135 Rat
- Omim: 605007 Human
- SwissProt: Q9UNA0 Human
- SwissProt: Q9R001 Mouse
- Unigene: 58324 Human
- Unigene: 112933 Mouse
see all -
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ADAMTS5 antibody (ab182795)Immunohistochemical analysis of paraffin embedded Human tonsil tissue labeling ADAMTS5 with ab182795 at 1/10. Image on the right is treated with the synthetic peptide.
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Western blot - Anti-ADAMTS5 antibody (ab182795)Anti-ADAMTS5 antibody (ab182795) at 1/650 dilution + Human fetal brain tissue at 40 µg
Secondary
Goat anti Rabbit IgG at 1/3000 dilution
Predicted band size: 102 kDa
Exposure time: 3 seconds
Datasheets and documents
References
ab182795 has not yet been referenced specifically in any publications.
