Anti-ADAMTS5 antibody (ab41037)
Key features and details
- Rabbit polyclonal to ADAMTS5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS5 antibody
See all ADAMTS5 primary antibodies -
Description
Rabbit polyclonal to ADAMTS5 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human ADAMTS5 aa 600-700 conjugated to keyhole limpet haemocyanin.
(Peptide available asab41036) -
Positive control
- ab41037 gave a positive signal in the following whole cell lysates: Uterus (Human)- normal tissue HeLa (Human epithelial carcinoma cell line) Placenta (Mouse) - normal tissue NIH 3T3 (Mouse embryonic fibroblast cell line) MEF1 (Mouse embryonic fibroblast cell line) PC12 (Rat adrenal pheochromocytoma cell line) ab41037 gave a positive signal in the following tissue lysates: Brain (Mouse),Liver (Mouse),Testis (Mouse),Spinal Cord (Mouse),Ovary (Mouse),Brain (Rat),Liver (Rat)
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab41037 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/250. Detects a band of approximately 73 kDa (predicted molecular weight: 102 kDa).Can be blocked with Human ADAMTS5 peptide (ab41036). ab41037 appears to detect the 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. |
Target
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Function
Cleaves aggrecan, a cartilage proteoglycan, and may be involved in its turnover. May play an important role in the destruction of aggrecan in arthritic diseases. May play a role in proteolytic processing mostly during the peri-implantation period. -
Tissue specificity
Expressed at low level in placenta primarily but also detected in heart and brain, cervix, uterus, bladder, esophagus, rib cartilage, chondroblastoma, fibrous tissue and a joint capsule from an arthritic patient. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 2 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsThe precursor is cleaved by a furin endopeptidase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 11096 Human
- Entrez Gene: 23794 Mouse
- Entrez Gene: 304135 Rat
- Omim: 605007 Human
- SwissProt: Q9UNA0 Human
- SwissProt: Q9R001 Mouse
- Unigene: 58324 Human
- Unigene: 112933 Mouse
see all -
Alternative names
- A disintegrin and metalloproteinase with thrombospondin motifs 11 antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 5 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 5 antibody
see all
Images
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Western blot - Anti-ADAMTS5 antibody (ab41037)All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lane 4 : Liver (Mouse) Tissue Lysate - normal tissue
Lane 5 : Testis (Mouse) Tissue Lysate - normal tissue
Lane 6 : Spinal Cord (Mouse) Tissue Lysate
Lane 7 : Ovary (Mouse) Tissue Lysate - normal tissue
Lane 8 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 9 : Brain (Rat) Tissue Lysate - normal tissue
Lane 10 : Liver (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?ab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
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Western blot - Anti-ADAMTS5 antibody (ab41037)All lanes : Anti-ADAMTS5 antibody (ab41037) at 1/250 dilution
Lane 1 : Human placenta tissue lysate - total protein (ab29745)
Lane 2 :Mouse placenta normal tissue lysate - total protein (ab29139)
Lane 3 : Uterus (Human) Whole Cell Lysate - normal tissue
Lane 4 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 27 kDa. We are unsure as to the identity of these extra bands.ab41037 appears to detect a 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase. The doublet observed in human uterus lysate is possibly due to glycosylation.
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Western blot - Anti-ADAMTS5 antibody (ab41037)All lanes : Anti-ADAMTS5 antibody (ab41037) at 1 µg/ml (blocked with 3% Milk)
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 12 minutesab41037 appears to detect at 73kDa cleaved form of ADAMTS5. The precursor of this secreted protein is cleaved at a furin cleavage site by a furin endopeptidase.
Protocols
References (62)
ab41037 has been referenced in 62 publications.
- Chen S et al. Moderate Fluid Shear Stress Regulates Heme Oxygenase-1 Expression to Promote Autophagy and ECM Homeostasis in the Nucleus Pulposus Cells. Front Cell Dev Biol 8:127 (2020). PubMed: 32195253
- Zhao X et al. MicroRNA-495 enhances chondrocyte apoptosis, senescence and promotes the progression of osteoarthritis by targeting AKT1. Am J Transl Res 11:2232-2244 (2019). PubMed: 31105831
- Zhou Y et al. Deletion of Axin1 in condylar chondrocytes leads to osteoarthritis-like phenotype in temporomandibular joint via activation of ß-catenin and FGF signaling. J Cell Physiol 234:1720-1729 (2019). PubMed: 30070692
- Xie WQ et al. Velvet antler polypeptide partially rescue facet joint osteoarthritis-like phenotype in adult ß-catenin conditional activation mice. BMC Complement Altern Med 19:191 (2019). PubMed: 31362725
- Pan X et al. Activation of Nrf2/HO-1 signal with Myricetin for attenuating ECM degradation in human chondrocytes and ameliorating the murine osteoarthritis. Int Immunopharmacol 75:105742 (2019). PubMed: 31325727
