Key features and details
- Rabbit polyclonal to ADAMTS7
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ADAMTS7 antibody
See all ADAMTS7 primary antibodies
DescriptionRabbit polyclonal to ADAMTS7
Specificityab201083 detects endogenous levels of ADAMTS7.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
- HEK293T, mouse Raw 264.7 and rat H9C2 cell lysates.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab201083 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Our Abpromise guarantee covers the use of ab201083 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 184 kDa.|
FunctionMetalloprotease that may play a role in the degradation of COMP.
Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 PLAC domain.
Contains 8 TSP type-1 domains.
DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsN-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed.
Cellular localizationSecreted, extracellular space, extracellular matrix. Also found associated with the external cell surface.
- Information by UniProt
- A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
ab201083 has not yet been referenced specifically in any publications.