Anti-ADAMTS7 antibody (ab203027)
Key features and details
- Rabbit polyclonal to ADAMTS7
- Suitable for: IHC-P
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS7 antibody
See all ADAMTS7 primary antibodies -
Description
Rabbit polyclonal to ADAMTS7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse ADAMTS7.
Database link: Q68SA9 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 48.98% TBS, 1X
Aqueous buffered solution -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab203027 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/100 - 1/500.
When using a fluorescent probe the recommended dilution is 1/50 – 1/200 |
Notes |
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IHC-P
1/100 - 1/500. When using a fluorescent probe the recommended dilution is 1/50 – 1/200 |
Target
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Function
Metalloprotease that may play a role in the degradation of COMP. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 PLAC domain.
Contains 8 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsN-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed. -
Cellular localization
Secreted, extracellular space, extracellular matrix. Also found associated with the external cell surface. - Information by UniProt
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Database links
- Entrez Gene: 315879 Rat
- SwissProt: Q1EHB3 Rat
- Unigene: 23850 Rat
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Alternative names
- A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
see all
Images
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded rat brain labeling ADAMTS7 with ab203027 at 1/200 dilution with DAB staining.
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Immunohistochemical analysis of Formalin-fixed, paraffin-embedded rat brain labeling ADAMTS7 with ab203027 at 1/200 dilution. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1/200 dilution for 40 minutes at 37°C.
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab203027 has been referenced in 6 publications.
- Ren W et al. Upregulation of miR-423 improves autologous vein graft restenosis via targeting ADAMTS-7. Int J Mol Med 45:532-542 (2020). PubMed: 31894258
- Wang C et al. ADAMTS7 degrades Comp to fuel BMP2-dependent osteogenic differentiation and ameliorate oncogenic potential in osteosarcomas. FEBS Open Bio 10:1856-1867 (2020). PubMed: 32692461
- Zhao X et al. MicroRNA-495 enhances chondrocyte apoptosis, senescence and promotes the progression of osteoarthritis by targeting AKT1. Am J Transl Res 11:2232-2244 (2019). PubMed: 31105831
- Hönig J et al. Transmission of microRNA antimiRs to mouse offspring via the maternal-placental-fetal unit. RNA 24:865-879 (2018). PubMed: 29540511
- Qin W et al. Upregulation of ADAMTS-7 and downregulation of COMP are associated with aortic aneurysm. Mol Med Rep 16:5459-5463 (2017). PubMed: 28849199
- Chen L et al. MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. J Cell Mol Med 21:3347-3359 (2017). WB . PubMed: 28609022