Overview

  • Product name

  • Description

    Rabbit polyclonal to ADAMTS7
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Mouse ADAMTS7.
    Database link: Q68SA9

  • Positive control

    • Rat brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab203027 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/1000. Predicted molecular weight: 182 kDa.
IHC-P 1/100 - 1/500.

When using a fluorescent probe the recommended dilution is 1/50 – 1/200

Target

  • Function

    Metalloprotease that may play a role in the degradation of COMP.
  • Tissue specificity

    Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.
  • Sequence similarities

    Contains 1 disintegrin domain.
    Contains 1 peptidase M12B domain.
    Contains 1 PLAC domain.
    Contains 8 TSP type-1 domains.
  • Domain

    The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    N-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
    May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed.
  • Cellular localization

    Secreted, extracellular space, extracellular matrix. Also found associated with the external cell surface.
  • Information by UniProt
  • Database links

  • Alternative names

    • A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
    • A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
    • A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
    • A disintegrin like and metalloprotease with thrombospondin type 1 motif 7 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 7 antibody
    • ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein antibody
    • ADAM TS 7 antibody
    • ADAM TS7 antibody
    • ADAM-TS 7 antibody
    • ADAM-TS7 antibody
    • ADAMTS 7 antibody
    • ADAMTS-7 antibody
    • Adamts7 antibody
    • ATS7_HUMAN antibody
    • COMPase antibody
    • DKFZp434H204 antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded rat brain labeling ADAMTS7 with ab203027 at 1/200 dilution with DAB staining.

  • Immunohistochemical analysis of Formalin-fixed, paraffin-embedded rat brain labeling ADAMTS7 with ab203027 at 1/200 dilution. The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated used at 1/200 dilution for 40 minutes at 37°C.

References

This product has been referenced in:

  • Qin W  et al. Upregulation of ADAMTS-7 and downregulation of COMP are associated with aortic aneurysm. Mol Med Rep 16:5459-5463 (2017). Read more (PubMed: 28849199) »
  • Chen L  et al. MiR-29b-3p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting PGRN. J Cell Mol Med 21:3347-3359 (2017). WB . Read more (PubMed: 28609022) »
See all 2 Publications for this product

Customer reviews and Q&As

Application
Western blot
Sample
Sheep Tissue lysate - whole (muscle)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
25 µg
Specification
muscle
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 25µg/mL · Temperature: 24°C

Abcam user community

Verified customer

Submitted May 26 2017

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