Anti-ADAMTS7 antibody (ab28557)
Key features and details
- Rabbit polyclonal to ADAMTS7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAMTS7 antibody
See all ADAMTS7 primary antibodies -
Description
Rabbit polyclonal to ADAMTS7 -
Host species
Rabbit -
Specificity
ab28557 recognizes metalloproteinase ADAMTS7. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ADAMTS7. Immunogen in the spacer-1 region of the C-terminus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Sucrose -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
The antibody has been peptide-affinity purified. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab28557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Notes |
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Target
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Function
Metalloprotease that may play a role in the degradation of COMP. -
Tissue specificity
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments. -
Sequence similarities
Contains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 PLAC domain.
Contains 8 TSP type-1 domains. -
Domain
The spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsN-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed. -
Cellular localization
Secreted, extracellular space, extracellular matrix. Also found associated with the external cell surface. - Information by UniProt
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Database links
- Entrez Gene: 11173 Human
- Omim: 605009 Human
- SwissProt: Q9UKP4 Human
- Unigene: 16441 Human
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Alternative names
- A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab28557 has been referenced in 6 publications.
- Pu X et al. Effect of a coronary-heart-disease-associated variant of ADAMTS7 on endothelial cell angiogenesis. Atherosclerosis 296:11-17 (2020). PubMed: 32005000
- Mu Y et al. Upregulation of ADAMTS-7 and downregulation of COMP are associated with spontaneous abortion. Mol Med Rep 19:2620-2626 (2019). PubMed: 30720083
- Yumusak N et al. Expression of ADAMTS-7 in myocardial dystrophy associated with white muscle disease in lambs. Pol J Vet Sci 21:119-126 (2018). PubMed: 29624002
- Chan K et al. Genetic Variation at theADAMTS7Locus is Associated With Reduced Severity of Coronary Artery Disease. J Am Heart Assoc 6:N/A (2017). IHC-P ; Human . PubMed: 29089340
- Wu W et al. Association of ADAMTS-7 Levels with Cardiac Function in a Rat Model of Acute Myocardial Infarction. Cell Physiol Biochem 38:950-8 (2016). PubMed: 26938210
- Pu X et al. ADAMTS7 cleavage and vascular smooth muscle cell migration is affected by a coronary-artery-disease-associated variant. Am J Hum Genet 92:366-74 (2013). PubMed: 23415669