Key features and details
- Rabbit polyclonal to ADAMTS7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADAMTS7 antibody
See all ADAMTS7 primary antibodies
DescriptionRabbit polyclonal to ADAMTS7
SpecificityThis antibody is specific for the prpetide domain after the second furin cleavage site of the full lenght ADAMTS7 and of the short form ADAMTS7A.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 2.9% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionMetalloprotease that may play a role in the degradation of COMP.
Tissue specificityExpressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Detected in meniscus, bone, tendon, cartilage, synovium, fat and ligaments.
Sequence similaritiesContains 1 disintegrin domain.
Contains 1 peptidase M12B domain.
Contains 1 PLAC domain.
Contains 8 TSP type-1 domains.
DomainThe spacer domain and the TSP type-1 domains are important for a tight interaction with the extracellular matrix.
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsN-glycosylated. Can be O-fucosylated by POFUT2 on a serine or a threonine residue found within the consensus sequence C1-X(2)-(S/T)-C2-G of the TSP type-1 repeat domains where C1 and C2 are the first and second cysteine residue of the repeat, respectively. Fucosylated repeats can then be further glycosylated by the addition of a beta-1,3-glucose residue by the glucosyltransferase, B3GALTL. Fucosylation mediates the efficient secretion of ADAMTS family members. Also can be C-glycosylated with one or two mannose molecules on tryptophan residues within the consensus sequence W-X-X-W of the TPRs. N- and C-glycosylations can also facilitate secretion. O-glycosylated proteoglycan. Contains chondroitin sulfate.
May be cleaved by a furin endopeptidase (By similarity). The precursor is sequentially processed.
Cellular localizationSecreted, extracellular space, extracellular matrix. Also found associated with the external cell surface.
- Information by UniProt
- A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein antibody
- A disintegrin and metalloproteinase with thrombospondin motifs 7 antibody
- A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7 antibody
All lanes : Anti-ADAMTS7 antibody (ab45044) at 1/1000 dilution
Lane 1 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 35 µl
Lane 2 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 30 µl
Lane 3 : Whole cell lysate prepared from human rhabdomyosarcoma RD cells (treated with TPA)
at 25 µl
Predicted band size: 181 kDa
ab45044 has been referenced in 3 publications.
- Jana S et al. Disparate Remodeling of the Extracellular Matrix and Proteoglycans in Failing Pediatric Versus Adult Hearts. J Am Heart Assoc 7:e010427 (2018). PubMed: 30371322
- Pu X et al. ADAMTS7 cleavage and vascular smooth muscle cell migration is affected by a coronary-artery-disease-associated variant. Am J Hum Genet 92:366-74 (2013). PubMed: 23415669
- Meliopoulos VA et al. MicroRNA regulation of human protease genes essential for influenza virus replication. PLoS One 7:e37169 (2012). WB ; Human . PubMed: 22606348