Anti-ADAMTSL4 antibody (ab71838)
Key features and details
- Mouse polyclonal to ADAMTSL4
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADAMTSL4 antibody
See all ADAMTSL4 primary antibodies -
Description
Mouse polyclonal to ADAMTSL4 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment (Human)
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Positive control
- ADAMTSL4 transfected 293T lysate; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab71838 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
ICC/IF |
Notes |
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Purify ab71838 with Protein A before proceeding with ICC/IF.
WB: 1/500 - 1/1000. Predicted molecular weight (calculated): 51 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Relevance
ADAMTSL4 is a member of ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs)-like family and has seven thrombospondin type 1 repeats. The thrombospondin type 1 repeat domain is found in many proteins with diverse biological functions including cellular adhesion, angiogenesis, and patterning of the developing nervous system. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. ADAMTSL4 is involved in the positive regulation of apoptosis. -
Cellular localization
Secreted -
Database links
- Entrez Gene: 54507 Human
- Omim: 610113 Human
- SwissProt: Q6UY14 Human
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Alternative names
- ADAMTS like 4 antibody
- ADAMTS like protein 4 antibody
- ADAMTSL 4 antibody
see all
Images
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Protein A purified ab71838 at 10µg/ml staining ADAMTSL4 in HeLa cells.
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All lanes : Anti-ADAMTSL4 antibody (ab71838) at 1/500 dilution
Lane 1 : ADAMTSL4 transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 51 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab71838 has not yet been referenced specifically in any publications.