Validated using a knockout cell line
Recombinant
RabMAb

Anti-ADAR1 antibody [EPR7033] (ab126745)

Overview

  • Product name
    Anti-ADAR1 antibody [EPR7033]
    See all ADAR1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7033] to ADAR1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ADAR1 aa 200-300. The exact sequence is proprietary.
    Database link: P55265

  • Positive control
    • HeLa, Ramos and SH-SY5Y cell lysates; Human brain tissue; HeLa cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab126745 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 136 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.
    • Tissue specificity
      Ubiquitously expressed, highest levels were found in brain and lung.
    • Involvement in disease
      Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
    • Sequence similarities
      Contains 1 A to I editase domain.
      Contains 2 DRADA repeats.
      Contains 3 DRBM (double-stranded RNA-binding) domains.
    • Post-translational
      modifications
      Sumoylation reduces RNA-editing activity.
    • Cellular localization
      Cytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.
    • Information by UniProt
    • Database links
    • Alternative names
      • 136 kDa double-stranded RNA-binding protein antibody
      • 136kDa double stranded RNA binding protein antibody
      • Adar 1 antibody
      • ADAR antibody
      • Adar1 antibody
      • Adenosine deaminase acting on RNA 1 A antibody
      • Adenosine deaminase RNA specific 1 antibody
      • Adenosine deaminase RNA specific antibody
      • Adenosine deaminase that act on RNA antibody
      • AGS6 antibody
      • AV242451 antibody
      • Double stranded RNA specific adenosine deaminase antibody
      • Double-stranded RNA-specific adenosine deaminase antibody
      • Double-stranded RNA-specific editase Adar antibody
      • DRADA antibody
      • Dsh antibody
      • Dsrad antibody
      • DSRAD_HUMAN antibody
      • dsRNA adenosine deaminase antibody
      • EC 3.5.4.- antibody
      • G1P1 antibody
      • IFI 4 antibody
      • IFI-4 antibody
      • IFI4 antibody
      • Ifi4 protein antibody
      • Interferon induced protein 4 antibody
      • Interferon inducible protein 4 antibody
      • Interferon-inducible protein 4 antibody
      • K88DSRBP antibody
      • mZaADAR antibody
      • P136 antibody
      • Pre-mRNA adenosine deaminase antibody
      • RNA adenosine deaminase 1 antibody
      • RNA-editing deaminase 1 antibody
      • RNA-editing enzyme 1 antibody
      see all

    Images

    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: ADAR1 knockout HAP1 cell lysate (20 µg)
      Lane 3: HepG2 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab126745 observed at 130 kDa. Red - loading control, ab8245, observed at 37 kDa.
      ab126745 was shown to recognize ADAR1 when ADAR1 knockout samples were used, along with additional cross-reactive bands. Wild-type and ADAR1 knockout samples were subjected to SDS-PAGE. ab126745 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-ADAR1 antibody [EPR7033] (ab126745) at 1/1000 dilution

      Lane 1 : HeLa (treated with IFN-alpha) cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Ramos cell lysate
      Lane 4 : SH-SY5Y cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 136 kDa

    • ab126745, at 1/50 dilution, staining ADAR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.
    • ab126745, at 1/50 dilution, staining ADAR1 in Hela cells by Immunofluorescence.
    • Flow cytometric analysis of permeabilized Ramos cells, staining ADAR1 (red) with ab126745.

      1x106 cells were collected and washed with blocking buffer. Cells were fixed with 2% paraformaldehyde, permeabilized with 1X FACS permeabilizing solution and blocked with blocking buffer for 30 minutes at room temperature. Cells were incubated with primary antibody (1/10) for 30 minutes at room temperature before a fluorescently-conjugated secondary antibody or 30 min at room temperature. A rabbit IgG was used as a negative control (green).

    References

    This product has been referenced in:
    • Lazzari E  et al. Alu-dependent RNA editing of GLI1 promotes malignant regeneration in multiple myeloma. Nat Commun 8:1922 (2017). Read more (PubMed: 29203771) »
    • Shi L  et al. Circular RNA expression is suppressed by androgen receptor (AR)-regulated adenosine deaminase that acts on RNA (ADAR1) in human hepatocellular carcinoma. Cell Death Dis 8:e3171 (2017). Read more (PubMed: 29144509) »

    See all 4 Publications for this product

    Customer reviews and Q&As

    Suite à notre conversation téléphonique, j’ai contacté le laboratoire à propos des résultats de cytométrie en flux pour ab126745 et ab126755.

    Les images peuvent être trouvées...

    Read More


    Unfortunately, we do not currently have any publicationfor https://www.abcam.com/ab126745. We will update the on-line datasheet for this product as soon as we receive additional data.

    The recommended protocol can be found via this link: h...

    Read More

    Thank you for contacting us.

    ab126755 is raised against a peptide from the C-terminal region (within last 50 amino acids) of isoform 1 of human ADAR1.
    ab126745 is raised against a peptide within the range from amino acid 200 to 250. Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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