Validated using a knockout cell line
Recombinant
RabMAb

Recombinant Anti-ADAR1 antibody [EPR7033] - BSA and Azide free (ab240029)

Overview

  • Product name

    Anti-ADAR1 antibody [EPR7033] - BSA and Azide free
    See all ADAR1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7033] to ADAR1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, IHC-P, WBmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human ADAR1 aa 200-300. The exact sequence is proprietary.
    Database link: P55265

  • General notes

    ab240029 is the carrier-free version of ab126745 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab240029 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab240029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Predicted molecular weight: 136 kDa.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Converts multiple adenosines to inosines and creates I/U mismatched base pairs in double-helical RNA substrates without apparent sequence specificity. Has been found to modify more frequently adenosines in AU-rich regions, probably due to the relative ease of melting A/U base pairs as compared to G/C pairs. Functions to modify viral RNA genomes and may be responsible for hypermutation of certain negative-stranded viruses. Edits the messenger RNAs for glutamate receptor (GLUR) subunits by site-selective adenosine deamination. Produces low-level editing at the GLUR-B Q/R site, but edits efficiently at the R/G site and HOTSPOT1. Binds to short interfering RNAs (siRNA) without editing them and suppresses siRNA-mediated RNA interference. Binds to ILF3/NF90 and up-regulates ILF3-mediated gene expression.
    • Tissue specificity

      Ubiquitously expressed, highest levels were found in brain and lung.
    • Involvement in disease

      Defects in ADAR are a cause of dyschromatosis symmetrical hereditaria (DSH) [MIM:127400]; also known as reticulate acropigmentation of Dohi. DSH is a pigmentary genodermatosis of autosomal dominant inheritance characterized by a mixture of hyperpigmented and hypopigmented macules distributed on the dorsal parts of the hands and feet.
    • Sequence similarities

      Contains 1 A to I editase domain.
      Contains 2 DRADA repeats.
      Contains 3 DRBM (double-stranded RNA-binding) domains.
    • Post-translational
      modifications

      Sumoylation reduces RNA-editing activity.
    • Cellular localization

      Cytoplasm. Nucleus > nucleolus. Isoform 1 is found predominantly in cytoplasm but appears to shuttle between the cytoplasm and nucleus. Isoform 5 is found exclusively in the nucleolus.
    • Information by UniProt
    • Database links

    • Alternative names

      • 136 kDa double-stranded RNA-binding protein antibody
      • 136kDa double stranded RNA binding protein antibody
      • Adar 1 antibody
      • ADAR antibody
      • Adar1 antibody
      • Adenosine deaminase acting on RNA 1 A antibody
      • Adenosine deaminase RNA specific 1 antibody
      • Adenosine deaminase RNA specific antibody
      • Adenosine deaminase that act on RNA antibody
      • AGS6 antibody
      • AV242451 antibody
      • Double stranded RNA specific adenosine deaminase antibody
      • Double-stranded RNA-specific adenosine deaminase antibody
      • Double-stranded RNA-specific editase Adar antibody
      • DRADA antibody
      • Dsh antibody
      • Dsrad antibody
      • DSRAD_HUMAN antibody
      • dsRNA adenosine deaminase antibody
      • EC 3.5.4.- antibody
      • G1P1 antibody
      • IFI 4 antibody
      • IFI-4 antibody
      • IFI4 antibody
      • Ifi4 protein antibody
      • Interferon induced protein 4 antibody
      • Interferon inducible protein 4 antibody
      • Interferon-inducible protein 4 antibody
      • K88DSRBP antibody
      • mZaADAR antibody
      • P136 antibody
      • Pre-mRNA adenosine deaminase antibody
      • RNA adenosine deaminase 1 antibody
      • RNA-editing deaminase 1 antibody
      • RNA-editing enzyme 1 antibody
      see all

    Images

    • ab126745, at 1/50 dilution, staining ADAR1 in paraffin-embedded Human brain tissue by Immunohistochemistry.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126745).

    • ab126745, at 1/50 dilution, staining ADAR1 in Hela cells by Immunofluorescence.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126745).

    • Flow cytometric analysis of permeabilized Ramos cells, staining ADAR1 (red) with ab126745.

      1x106 cells were collected and washed with blocking buffer. Cells were fixed with 2% paraformaldehyde, permeabilized with 1X FACS permeabilizing solution and blocked with blocking buffer for 30 minutes at room temperature. Cells were incubated with primary antibody (1/10) for 30 minutes at room temperature before a fluorescently-conjugated secondary antibody or 30 min at room temperature. A rabbit IgG was used as a negative control (green).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126745).

    References

    ab240029 has not yet been referenced specifically in any publications.

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