Anti-ADCK3 antibody (ab230897)
Key features and details
- Rabbit polyclonal to ADCK3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ADCK3 antibody -
Description
Rabbit polyclonal to ADCK3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Synthetic peptide corresponding to Human ADCK3 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q8NI60 -
Positive control
- WB: HeLa and U-251 MG whole cell lysates. IHC-P: Human thymus tissue. ICC/IF: HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100.
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WB |
1/500 - 1/1000. Predicted molecular weight: 72 kDa.
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ICC/IF |
1/100 - 1/500.
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Notes |
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IHC-P
1/100. |
WB
1/500 - 1/1000. Predicted molecular weight: 72 kDa. |
ICC/IF
1/100 - 1/500. |
Target
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Function
May be a chaperone-like protein essential for the proper conformation and functioning of protein complexes in the respiratory chain. -
Tissue specificity
Ubiquitously expressed with a relatively greater abundance in heart and skeletal muscle. -
Involvement in disease
Defects in ADCK3 are a cause of coenzyme Q10 deficiency (CoQ10 deficiency) [MIM:607426]. CoQ10 deficiency is an autosomal recessive disorder with variable manifestations. It can be associated with three main clinical phenotypes: a predominantly myopathic form with central nervous system involvement, an infantile encephalomyopathy with renal dysfunction and an ataxic form with cerebellar atrophy.
Defects in ADCK3 are the cause of spinocerebellar ataxia autosomal recessive type 9 (SCAR9) [MIM:612016]; also known as autosomal recessive cerebellar ataxia type 2 (ARCA2). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR9 is an autosomal recessive form characterized by gait ataxia and cerebellar atrophy with slow progression and few associated features. Patients can manifest brisk tendon reflexes and Hoffmann sign, mild psychomotor retardation, mild axonal degeneration of the sural nerve, exercise intolerance and elevated serum lactate. -
Sequence similarities
Belongs to the protein kinase superfamily. ADCK protein kinase family.
Contains 1 protein kinase domain. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 536925 Cow
- Entrez Gene: 56997 Human
- Entrez Gene: 67426 Mouse
- Entrez Gene: 360887 Rat
- Omim: 606980 Human
- SwissProt: Q29RI0 Cow
- SwissProt: Q8NI60 Human
- SwissProt: Q60936 Mouse
see all -
Alternative names
- aarF domain containing protein kinase 3 antibody
- aarF domain-containing protein kinase 3 antibody
- ADCK 3 antibody
see all
Images
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All lanes : Anti-ADCK3 antibody (ab230897) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Predicted band size: 72 kDa -
Formalin-fixed, paraffin-embedded human thymus tissue stained for ADCK3 using ab230897 at 1/100 dilution in immunohistochemical analysis.
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Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ADCK3 (red) using ab230897 at 1/100 dilution followed by DyLight 594-congugated secondary antibody in ICC/IF.
The nuclear counter stain is DAPI (blue).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab230897 has been referenced in 1 publication.
- Zhuo BB et al. ADCK1 is a potential therapeutic target of osteosarcoma. Cell Death Dis 13:954 (2022). PubMed: 36371387