Key features and details
- Rabbit polyclonal to ADCK3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADCK3 antibody
See all ADCK3 primary antibodies
DescriptionRabbit polyclonal to ADCK3
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Synthetic peptide corresponding to Human ADCK3 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: Q8NI60
- WB: HeLa and U-251 MG whole cell lysates. IHC-P: Human thymus tissue. ICC/IF: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Predicted molecular weight: 72 kDa.|
|ICC/IF||1/100 - 1/500.|
FunctionMay be a chaperone-like protein essential for the proper conformation and functioning of protein complexes in the respiratory chain.
Tissue specificityUbiquitously expressed with a relatively greater abundance in heart and skeletal muscle.
Involvement in diseaseDefects in ADCK3 are a cause of coenzyme Q10 deficiency (CoQ10 deficiency) [MIM:607426]. CoQ10 deficiency is an autosomal recessive disorder with variable manifestations. It can be associated with three main clinical phenotypes: a predominantly myopathic form with central nervous system involvement, an infantile encephalomyopathy with renal dysfunction and an ataxic form with cerebellar atrophy.
Defects in ADCK3 are the cause of spinocerebellar ataxia autosomal recessive type 9 (SCAR9) [MIM:612016]; also known as autosomal recessive cerebellar ataxia type 2 (ARCA2). Spinocerebellar ataxia is a clinically and genetically heterogeneous group of cerebellar disorders. Patients show progressive incoordination of gait and often poor coordination of hands, speech and eye movements, due to degeneration of the cerebellum with variable involvement of the brainstem and spinal cord. SCAR9 is an autosomal recessive form characterized by gait ataxia and cerebellar atrophy with slow progression and few associated features. Patients can manifest brisk tendon reflexes and Hoffmann sign, mild psychomotor retardation, mild axonal degeneration of the sural nerve, exercise intolerance and elevated serum lactate.
Sequence similaritiesBelongs to the protein kinase superfamily. ADCK protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- aarF domain containing protein kinase 3 antibody
- aarF domain-containing protein kinase 3 antibody
- ADCK 3 antibody
All lanes : Anti-ADCK3 antibody (ab230897) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Predicted band size: 72 kDa
Formalin-fixed, paraffin-embedded human thymus tissue stained for ADCK3 using ab230897 at 1/100 dilution in immunohistochemical analysis.
Formalin-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ADCK3 (red) using ab230897 at 1/100 dilution followed by DyLight 594-congugated secondary antibody in ICC/IF.
The nuclear counter stain is DAPI (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230897 has not yet been referenced specifically in any publications.