Product nameAnti-ADCK4 antibody
See all ADCK4 primary antibodies
DescriptionRabbit polyclonal to ADCK4
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ADCK4 aa 10-137.
Database link: Q96D53
- WB: HeLa, A549 and HepG2 whole cell lysate. IHC-P: Human kidney tissue and melanoma cancer tissues. ICC/IF: A549 cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.03% Proclin
Constituents: 50% Glycerol, PBS
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 60, 56 kDa (predicted molecular weight: 60 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionThe function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).
Sequence similaritiesBelongs to the protein kinase superfamily. ADCK protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- aarF domain containing protein kinase 4 antibody
- ADCK4 antibody
- ADCK4_HUMAN antibody
All lanes : Anti-ADCK4 antibody (ab223114) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 56,60 kDa why is the actual band size different from the predicted?
A549 (human lung carcinoma cell line) cells stained for ADCK4 (green) using ab223114 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human melanoma cancer tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.
ab223114 has not yet been referenced specifically in any publications.