Key features and details
- Rabbit polyclonal to ADCK4
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-ADCK4 antibody
See all ADCK4 primary antibodies
DescriptionRabbit polyclonal to ADCK4
Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Recombinant fragment corresponding to Human ADCK4 aa 10-137.
Database link: Q96D53
- WB: HeLa, A549 and HepG2 whole cell lysate. IHC-P: Human kidney tissue and melanoma cancer tissues. ICC/IF: A549 cells.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Constituents: 50% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300
Concentration information loading...
PurityProtein G purified
Purification notesPurity >95%.
Our Abpromise guarantee covers the use of ab223114 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/50 - 1/200.|
|WB||1/500 - 1/5000. Detects a band of approximately 60, 56 kDa (predicted molecular weight: 60 kDa).|
|IHC-P||1/20 - 1/200.|
FunctionThe function of this protein is not yet clear. It is not known if it has protein kinase activity and what type of substrate it would phosphorylate (Ser, Thr or Tyr).
Sequence similaritiesBelongs to the protein kinase superfamily. ADCK protein kinase family.
Contains 1 protein kinase domain.
- Information by UniProt
- aarF domain containing protein kinase 4 antibody
- ADCK4 antibody
- ADCK4_HUMAN antibody
All lanes : Anti-ADCK4 antibody (ab223114) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 60 kDa
Observed band size: 56,60 kDa why is the actual band size different from the predicted?
A549 (human lung carcinoma cell line) cells stained for ADCK4 (green) using ab223114 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Paraffin-embedded human melanoma cancer tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney tissue stained for ADCK4 using ab223114 at 1/100 dilution in immunohistochemical analysis.
ab223114 has not yet been referenced specifically in any publications.