Key features and details
- Rabbit polyclonal to ADCY10/SAC
- Suitable for: IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-ADCY10/SAC antibody
DescriptionRabbit polyclonal to ADCY10/SAC
Tested applicationsSuitable for: IHC-Pmore details
Species reactivityReacts with: Mouse, Rat, Human
This product was previously labelled as ADCY10
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 50% Glycerol, 0.01% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab203204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use at 1/50 - 1/200 with fluorescent detection methods.
RelevanceADCY10 belongs to a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or forskolin regulation. It is localized in the cytoplasm and is thought to function as a general bicarbonate sensor throughout the body. It may also play an important role in the generation of cAMP in spermatozoa, implying possible roles in sperm maturation through the epididymis, capacitation, hypermotility, and/or the acrosome reaction.
Cellular localizationCell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin.
- 3',5'-cyclic AMP synthetase antibody
- Adenylate cyclase 10 (soluble) antibody
- Adenylate cyclase 10 antibody
Immunohistochemistry on Formalin/PFA-fixed paraffin-embedded rat brain tissue labeling ADCY10/SAC with ab203204 at a 1/200 dilution, followed by conjugation to the secondary antibody Goat anti-rabbit IgG-Cy3 at a 1/200 dilution.
Immunohistochemistry on Formalin/PFA-fixed paraffin-embedded rat lung tissue labeling ADCY10/SAC with ab203204 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.
Immunohistochemistry on Formalin/PFA-fixed paraffin-embedded human liver cancer tissue labeling ADCY10/SAC with ab203204 at a 1/100 dilution, followed by conjugation to the secondary antibody and DAB staining.
ab203204 has been referenced in 2 publications.
- Shaw PX et al. Soluble Adenylyl Cyclase Is Required for Retinal Ganglion Cell and Photoreceptor Differentiation. Invest Ophthalmol Vis Sci 57:5083-5092 (2016). IHC-Fr ; Mouse . PubMed: 27679853
- Nixon B et al. The Australian saltwater crocodile (Crocodylus porosus) provides evidence that the capacitation of spermatozoa may extend beyond the mammalian lineage. Proc Biol Sci 283:N/A (2016). PubMed: 27147099