Product nameAnti-ADCY10/SAC antibody
See all ADCY10/SAC primary antibodies
DescriptionRabbit polyclonal to ADCY10/SAC
Tested applicationsSuitable for: IHC-Fr, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog
- Human fetal brain lysate
This product was previously labelled as ADCY10
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified by peptide affinity chromatography method.
Our Abpromise guarantee covers the use of ab82854 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration. PubMed: 22649251|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 187 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.|
RelevanceADCY10 belongs to a distinct class of mammalian adenylyl cyclase that is soluble and insensitive to G protein or forskolin regulation. It is localized in the cytoplasm and is thought to function as a general bicarbonate sensor throughout the body. It may also play an important role in the generation of cAMP in spermatozoa, implying possible roles in sperm maturation through the epididymis, capacitation, hypermotility, and/or the acrosome reaction.
Cellular localizationCell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Cytoplasm, perinuclear region. Note: Distributed to subcellular compartments containing cAMP targets. Found as a plasma membrane associated protein, protein concentrated in the perinuclear region and protein colocalized with actin or tubulin.
- 3',5'-cyclic AMP synthetase antibody
- Adenylate cyclase 10 (soluble) antibody
- Adenylate cyclase 10 antibody
Anti-ADCY10/SAC antibody (ab82854) at 1 µg/ml (in 5% skim milk / PBS buffer) + Fetal Brain lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 187 kDa
Observed band size: 145 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Gel concentration 6-18%
This product has been referenced in:
- Shaw PX et al. Soluble Adenylyl Cyclase Is Required for Retinal Ganglion Cell and Photoreceptor Differentiation. Invest Ophthalmol Vis Sci 57:5083-5092 (2016). WB ; Mouse . Read more (PubMed: 27679853) »
- Corredor RG et al. Soluble adenylyl cyclase activity is necessary for retinal ganglion cell survival and axon growth. J Neurosci 32:7734-44 (2012). IHC-Fr ; Rat . Read more (PubMed: 22649251) »